Marker, immunoassay method, immunoassay reagent, method for assaying analyte, analyte measurement kit, and lateral-flow chromatographic test strip
View Patent ↗A resin-metal composite 100 to be used as a marker in an immunoassay, said resin-metal composite 100 comprising a resin particle 10 and metal particles 20 and having the following constitution (A) or constitution (B): (A) the average particle size of the resin-metal composite exceeding 300 nm; or (B) the average particle size of the metal particles being in the range of more than 20 nm and less than 70 nm. It is preferred that a part of the metal particles 20 are two- or three-dimensionally distributed in the surface layer part 60 of the resin particle 10 , a part of the three-dimensionally distributed metal particles 20 are partly exposed to the outside of the resin particle 10 , and a part of the remainder particles are enclosed in the resin particle 10.
1. A marker, comprising a resin-gold composite with a structure formed by immobilizing old particles on a resin particle, characterized by
having
the average particle size of the resin-gold composite being in a range of 300 nm to 1000 nm;
the average particle size of the gold particles being in a range of 20 nm to 70 nm;
wherein the gold particles comprise
a first portion of the gold particles completely encased in the resin particle,
a second portion of the gold particles having a part embedded in the resin particle and another part exposed from the resin particle, and
a remaining portion of the gold particles adsorbed on the surface of the resin particle;
wherein 75% to 100% of the gold particles exist in a range of 50% of particle radius in a depth direction from a surface of the resin particle;
wherein the resin particle comprises a nitrogenous polymer having nitrogen ions on the main chain or the side chain, and the nitrogenous polymer is selected from the group consisting of poly-2-vinylpyridine, poly-3-vinylpyridine, poly-4-vinylpyridine and 2-(diisopropylamino)ethyl methacrylate.
2. The marker according to claim 1 , wherein the resin-gold composite is dispersed in water.
3. The marker according to claim 1 , wherein the marker is used in adsorbing an antigen or an antibody on the surface of the resin-gold composite.
4. An immunoassay method, characterized by using the marker according to claim 1 .
5. An immunoassay reagent, comprising the marker according to claim 1 .
6. A method for measuring analyte, assaying or quantifying an analyte contained in a sample, characterized by
using a lateral-flow chromatographic test strip comprising a membrane and a determination portion formed by immobilizing capturing ligands specifically bound with the analyte on the membrane to carry out steps including step (I) to step (II) hereinafter:
step (I): step of making the analyte contained in the sample contact with a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte;
step (II): step of making the composite containing the analyte and the marked antibody formed in step (I) contact with the capturing ligands in the determination portion; and
step (III): step of determining colored intensity derived from the localized surface plasmon resonance of the resin-gold composite in the marker.
7. An analyte measurement kit, using a lateral-flow chromatographic test strip, used to assay or quantify an analyte contained in a sample, used to assay or quantify the analyte, and the analyte measurement kit comprising:
the lateral-flow chromatographic test strip, comprising a membrane and a determination portion formed by immobilizing capturing ligands specifically bound with the analyte on the membrane; and
an assay reagent, containing a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte.
8. A lateral-flow chromatographic test strip, used to assay or quantify an analyte contained in a sample, comprising
a membrane;
a determination portion, formed by immobilizing capturing ligands specifically bound with the analyte on the membrane in the spreading direction of a sample; and
a reaction portion, comprising a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte further upstream than the determination portion.