Biosynthesis and engineering of lanthipeptides
Platforms, systems and methods are provided for identifying engineered lanthipeptide display peptides expressed in vivo from biological organisms.
1. A lanthipeptide display system, comprising:
a gene chimera encoding a fusion peptide comprising a lanthipeptide display peptide and a presentation peptide,
wherein the presentation peptide anchors the lanthipeptide display peptide on an outer biological surface,
wherein the lanthipeptide display peptide comprises a lanthionine amino acid moiety, wherein the lanthipeptide display peptide or presentation peptide comprises SEQ ID NOs.: 9, 75 and 76.
2. The lanthipeptide display system of claim 1 , wherein the outer biological surface is selected from a phage surface or a host organism membrane.
3. The lanthipeptide display system of claim 1 , wherein the gene chimera encoding a fusion peptide comprising a lanthipeptide display peptide and a presentation peptide is expressed from one of a phagemid, a plasmid, a cosmid, and a chromosome.
4. A lanthipeptide library display system, comprising:
a lanthipeptide expression library comprising a plurality of gene chimeras, wherein each member of the plurality of gene chimeras encodes a fusion peptide comprising a lanthipeptide display peptide and a presentation peptide,
wherein the presentation peptide anchors the lanthipeptide display peptide on an outer biological surface,
wherein the lanthipeptide display peptide comprises a lanthionine amino acid moiety,
wherein the lanthipeptide display peptide or presentation peptide comprises SEQ ID NOs.: 9, 75 and 76.
5. The lanthipeptide library display system of claim 4 , wherein the outer biological surface is selected from a phage surface or a host organism membrane.
6. The lanthipeptide library display system of claim 4 , wherein each member of the plurality of gene chimeras is expressed from a uniform lanthipeptide expression library selected from one system consisting of a phagemid, a plasmid, a cosmid and a chromosome.