LIPO-CHITOOLIGOSACCHARIDE COMPOSITIONS FOR ENHANCING PLANT GROWTH
The application relates to compositions for enhancing plant growth, comprising at least one lipo-chitooligosaccharide (LCO) compound; and one beneficial microorganism selected from Streptomyces, Trichoderma or Bacillus and optionally further pesticide and to the methods for enhancing plant growth and treating seeds with said compositions.
1 . A method for synergistically enhancing the growth of a plant or plant part, comprising contacting said plant or plant part with at least one lipo-chitooligosaccharide (LCO) and at least one agriculturally beneficial microorganism, said at least one agriculturally beneficial microorganism comprising Bacillus sp., isolat AQ175, ATCC 55608; Bacillus sp., isolate AQ177, ATCC 55609; Bacillus amyloliquefaciens FZB24; Baccillus amyloliquefaciens isolate NRRL B-50349; Bacillus amyloliquefaciens SB3778; Bacillus amyloliquefaciens TJ1000; Bacillus pumilus isolate AQ717, NRRL B-21662; Bacillus Punilus isolate NRRL B-30087; Bacillus subtilis isolate AQ713, NRRL V-21661; Bacillus subtilis isolate AQ743, NRRL B-21665; Bacillus thuringiensis isolate AQ52, NRRL B-21619; Streptomyces sp. isolate NRRL No. B-30145, Striptomyces sp. isolate M1064, Streptomyces WYE 53, Streptomyces galbus isolate NRRL 30232, Streptomyces lydicus WYEC 108, Streptomyces violaceusniger YCED 9, Trichoderma asperelium SKT-1, Trichoderma atroviride LC52, Trichoderma harzianum T-22, Trichoderma harzianum TJ-35, Trichoderma harzianum T-39, Trichoderma harzianum ICC012, Trichoderma virens GL-21, Trichoderma virens GI-3, Trichoderma virens GI-21, Trichoderma viride TV1, and/or Trichoderma viride ICC080.
2 . The method of claim 1 , wherein said at least one LCO comprises one or more LCOs represented by the structure (I):
in which G is selected from the group consisting of hexosamine, acetyl-substituted hexosamine, sulfated-substituted hexosamine, and ether-subsititued hexosamine; R 1 , R 2 , R 3 , R 5 , R 6 and R 7 , which are identical or different, are selected from the group consisting of H, CH 3 CO—, C x H y CO—, a carbamoyl, and a carbamic acid, wherein x is an integer between 0 and 17, and y is an integer between 1 and 35; R 4 is selected from the group consisting of a saturated, monounsaturated, diunsaturated or triunsaturated aliphatic chain containing at least 12 carbon atoms; and n is equal to 1, 2, 3, or 4.
3 . The method of claim 1 , wherein said at least one LCO comprises one or more LCOs represented by the structure (II):
in which R is H or CH 3 CO—; and n is equal to 2 or 3.
4 . The method of claim 1 , wherein said at least one LCO comprises one or more LCOs represented by the structure (III):
in which R 1 is selected from the group consisting of C14:0, 3OH-C14:0, iso-C15:0, C16:0, 3-OH-016:0, iso-C15:0, C16:1, C16:2, C16:3, iso-C17:0, iso-C17:1, C18:0, 3OH-C18:0, 018:0/3-OH, C18:1, OH-C18:1, C18:2, C18:3, C18:4, C19:1 carbamoyl, C20:0, C20:1, 3-OH-C20:1, 020:1/3-OH, C20:2, C20:3, C22:1, and C18-26(ω-1)-OH (including the C18, C20, C22, C24 and C26 hydroxylated species thereof, and C16:1Δ9, C16:2 (Δ2,9) and C16:3 (Δ2,4,9)); R 2 hydrogen or methyl; R 3 is selected from the group consisting of hydrogen, acetyl or carbamoyl; R 4 hydrogen, acetyl or carbamoyl; R 5 represents hydrogen, acetyl or carbamoyl; R 6 is selected from the group consisting of hydrogen, arabinosyl, fucosyl, acetyl, sulfate ester, SO 3 H, 3-0-S-2-0-MeFuc, 2-0-MeFuc, and 4-0-AcFuc; R 7 is selected from the group consisting of hydrogen, mannosyl or glycerol; R 8 is selected from the group consisting of hydrogen, methyl, or —CH 2 OH; R 9 is selected from the group consisting of hydrogen, arabinosyl, or fucosyl; R 10 is selected from the group consisting of hydrogen, acetyl or fucosyl; and n is equal to 0, 1, 2 or 3.
5 . The method of claim 1 , wherein said at least one LCO comprises one or more LCOs represented by the structure (IV):
in which R 1 is selected from the group consisting of C16, C16:0, C16:1, C16:2, C18:0, C18:1Δ9Z or C18:1Δ11Z; R 2 represents hydrogen or SO 3 H; and n is equal to 1 or 2.
6 . The composition of claim 1 , wherein said at least one LCO comprises:
an LCO represented by the structure (V):
an LCO represented by the structure (VI):
an LCO represented by the structure (VII):
an LCO represented by the structure (VIII):
an LCO represented by the structure (IX):
an LCO represented by the structure (X):
an LCO represented by the structure (XI):
an LCO represented by the structure (XII):
an LCO represented by the structure (XIII):
an LCO represented by the structure (XIV):
an LCO represented by the structure (XV):
an LCO represented by the structure (XVI):
an LCO represented by the structure (XVII):
an LCO represented by the structure (XVIII):
an LCO represented by the structure (XIX):
an LCO represented by the structure (XX):
an LCO represented by the structure (XXI):
an LCO represented by the structure (XXII):
an LCO represented by the structure (XXIII):
an LCO represented by the structure (XXIV):
an LCO represented by the structure ( )W):
an LCO represented by the structure (XXVI):
an LCO represented by the structure (XXVII):
an LCO represented by the structure (XXVIII):
an LCO represented by the structure (XXIX):
an LCO represented by the structure (XXX):
an LCO represented by the structure (XXXI):
an LCO represented by the structure (XXXII):
and/or an LCO represented by the structure (XXXIII):
7 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Bacillus amyloliquefaciens FZB24.
8 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Bacillus amyloliquefaciens SB3778.
9 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Bacillus amyloliquefaciens TJ1000.
10 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Streptomyces lydicus WYEC 108.
11 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Trichoderma harzianum ICC012.
12 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Trichoderma virens GI-3.
13 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Trichoderma virens GI-21.
14 . The method of claim 1 , wherein said at least one agriculturally beneficial microorganism comprises Trichoderma viride ICC080.
15 . The method of claim 1 , further comprising contacting said plant or plant part with at least one fungicide, herbicie, insecticide, acaricide, and/or nematicide.
16 . The method of claim 1 , further comprising contacting said plant or plant part with azoxystrobin, pyraoxystrobin, penflufen, sedaxane, dimethomorph and/or ipconazole.
17 . The method of any claim 1 , further comprising contacting said plant or plant part with lambda-cyhalothrin, gamma-cyhalothrin, imidacloprid, thiamethoxam, pyriprole, chlorantraniliprole, clothianidin, cyazypyr, abamectin, and/or pyrimidifen
18 . (canceled)