Heterogeneous inseminate system
A first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species of animal for the production of sex-selected embryos and sex-selected offspring.
1. A method of producing an inseminate, comprising:
obtaining a first amount of sperm of an animal, said first amount of sperm containing X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells;
obtaining a second amount of sperm of said animal, said second amount of sperm comprising an isolated subpopulation of X-chromosome bearing sperm cells or an isolated subpopulation of Y-chromosome bearing sperm cells of said animal; and
combining said first amount of sperm of said animal with said second amount of sperm of said animal;
adjusting concentration of said first amount of sperm in said inseminate, said concentration of said first amount of sperm in said inseminate sufficient to increase motility or fertility of said second amount of sperm in said inseminate.
2. The method of claim 1 , wherein said first amount of sperm has a greater concentration in said inseminate than said second amount of sperm in said inseminate.
3. The method of claim 1 , wherein said animal is selected from the group consisting of: an ayes, and a fish.
4. The method of claim 1 , wherein said animal comprises a bovine mammal and further comprising establishing a concentration of said second amount of sperm of said animal in said inseminate at about 2×10 4 sperm per milliliter to about 4×10 6 sperm per milliliter.
5. The method of claim 4 , further comprising establishing concentration of said first amount of sperm of said animal in said heterogeneous inseminate at about 2×10 7 sperm per milliliter to about 8×10 7 sperm per milliliter.
6. The method of claim 5 , further comprising establishing said heterogeneous inseminate in a dosage form having a volume of about one-quarter milliliter to about one milliliter contained in an artificial insemination straw.
7. The method of claim 6 , further comprising freezing said dosage form having said volume of about one-quarter milliliter to about one milliliter contained in said artificial insemination straw.
8. The method of claim 1 , wherein said first amount of sperm comprises infertile sperm.
9. The method of claim 1 , wherein said first amount of sperm or said second amount of sperm comprises frozen-thawed sperm.
10. The method of claim 1 , further comprising admixing an amount of cell free seminal fluid with said first amount of sperm and said second amount of sperm.
11. The method of claim 1 , wherein said isolated subpopulation of X-chromosome bearing sperm cells or said isolated subpopulation of Y-chromosome bearing sperm cells of said second amount of sperm has a non-naturally occurring ratio of X-chromosome bearing sperm in or Y-chromosome bearing sperm.
12. The method of claim 11 , wherein said first amount of sperm further comprises an isolated subpopulation of X-chromosome bearing sperm cells or an isolated subpopulation of Y-chromosome bearing sperm cells of said first amount of sperm of said animal.
13. The method of claim 12 , wherein said isolated subpopulation of X-chromosome bearing sperm cells or said isolated subpopulation of Y-chromosome bearing sperm cells of said first amount of sperm and said second amount of sperm each have a non-naturally occurring ratio of X-chromosome bearing sperm or Y-chromosome bearing sperm.
14. The method of claim 13 , wherein said isolated subpopulation of X-chromosome bearing sperm cells or said isolated subpopulation of Y-chromosome bearing sperm cells of said first amount of sperm or said second amount of sperm contain DNA binding an amount of stain.
15. The method of claim 1 , further comprising obtaining said first amount of sperm from a first animal and obtaining said second amount of sperm from a second animal, said first animal and said second animal belonging to one species.
16. The method of claim 1 , wherein said first amount of sperm comprises only infertile sperm.