Processes for producing hydrocarbon products
The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.
1. A process for producing extracted plant lipid, the process comprising the steps of:
i) extracting lipid from a collection of transgenic vegetative plant parts having a total non-polar lipid content of at least 10% (w/w dry weight); and
ii) recovering the extracted lipid.
2. The process of claim 1 , wherein the step of extracting the lipid comprises one or more rolling, pressing, crushing or grinding the vegetative plant parts.
3. The process of claim 1 , further comprising a step of converting the extracted lipid to an industrial product by applying heat, chemical means, or enzymatic means, or any combination thereof to the extracted lipid.
4. The process of claim 3 , in which at least some of the lipid is converted to the industrial product by chemical means, wherein i) the chemical means comprises reacting the non-polar lipid with an alcohol, optionally in the presence of a catalyst, to produce alkyl esters, and ii) optionally, blending the alkyl esters of step i) with petroleum based fuel.
5. The process of claim 1 , wherein step (i) uses an organic solvent.
6. The process of claim 5 , wherein the organic solvent comprises hexane, diethyl ether, petroleum ether, chloroform/methanol, butanol or benzene.
7. The process of claim 1 which comprises one or more or all of:
a) recovering the extracted lipid by collecting it in a container,
b) one or more of degumming, deodorising, decolourising, drying or fractionating the extracted lipid,
c) removing at least some waxes and/or wax esters from the extracted lipid, and
d) analysing the fatty acid composition of the extracted lipid.
8. The process of claim 1 , wherein the vegetative plant parts are plant leaves.
9. The process of claim 1 , wherein the vegetative plant parts have one or more or all of the following features:
the total fatty acid content in the non-polar lipid in the vegetative plant parts comprises 19% oleic acid,
the total fatty acid content in the non-polar lipid in the vegetative plant parts comprises 20% palmitic acid,
the total fatty acid content in the non-polar lipid in the vegetative plant parts comprises 15% linoleic acid, and
the total fatty acid content in the non-polar lipid in the vegetative plant parts comprises less than 15% α-linoleic acid.
10. The process of claim 1 , wherein the total fatty acid content in the non-polar lipid in the vegetative plant parts comprises 19% oleic acid.
11. The process of claim 10 , wherein the vegetative plant parts are plant leaves.
12. The process of claim 1 , wherein the vegetative plant parts have a total non-polar lipid content of about 11% (w/w dry weight).
13. The process of claim 1 , wherein the vegetative plant parts have a total non-polar lipid content of about 15% (w/w dry weight).
14. The process of claim 13 , wherein the vegetative plant parts are plant leaves.
15. The process of claim 11 , wherein the vegetative plant parts comprise a total TAG content of about 11% (w/w dry weight).
16. The process of claim 1 , wherein the total fatty acid content in the non-polar lipid of the vegetative plant parts comprises 2% more oleic acid than the non-polar lipid in a corresponding wild-type vegetative plant part.
17. The process of claim 1 , wherein the total fatty acid content in the non-polar lipid of the vegetative plant parts comprises 2% less palmitic acid than the non-polar lipid in a corresponding wild-type vegetative plant part.
18. The process of claim 1 , wherein the non-polar lipid of the vegetative plant parts comprises a modified level of total sterols, non-esterified sterols, steroyl esters or steroyl glycosides relative to the non-polar lipid in a corresponding wild-type vegetative plant part.
19. The process of claim 2 which comprises a step of harvesting the vegetative plant parts from plants grown in the field with a mechanical harvester.
20. The process of claim 19 , wherein the vegetative plant parts are harvested from the plants at a time between about the time of flowering of the plants to about the time senescence of the plants has started.
21. A process for producing a feedstuff, the process comprising admixing the extracted plant lipid produced in claim 1 with at least one other food ingredient.