Lettuce variety 45-140 RZ

1. An iceberg lettuce plant designated 45-140 RZ, representative seed of which having been deposited under NCIMB Accession No. 42471, wherein said plant comprises at least the following combination of traits including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf.

2. A seed of the plant of claim 1 .

3. A part of the plant of claim 1 , wherein said part of the plant is suitable for sexual reproduction.

4. The part of the plant as claimed in claim 3 , wherein said part comprises a microspore, pollen, an ovary, an ovule, an embryo sac or an egg cell.

5. A part of the plant of claim 1 , wherein said part of the plant is suitable for vegetative reproduction.

6. The part of the plant as claimed in claim 5 , wherein said part comprises a cutting, a root, a stem, a cell or a protoplast.

7. A tissue culture of regenerable cells or protoplasts from the lettuce plant of claim 1 .

8. The tissue culture as claimed in claim 7 , wherein said cells or protoplasts of the tissue culture are derived from a leaf, pollen, an embryo, a cotyledon, a hypocotyl, a meristematic cell, a root, a root tip, an anther, a flower, a seed or a stem.

9. A method for producing a progeny plant of a lettuce plant of claim 1 , comprising crossing the plant of claim 1 with itself or with another lettuce plant, harvesting the resultant seed, and growing said seed.

10. A progeny plant produced by the method of claim 9 , wherein said progeny plant exhibits a combination of traits including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf.

11. The progeny plant as claimed in claim 10 , wherein said progeny plant is modified in one or more other characteristics.

12. The progeny plant as claimed in claim 11 , wherein the modification is effected by mutagenesis.

13. The progeny plant as claimed in claim 11 , wherein the modification is effected by transformation with a transgene.

14. A progeny plant produced by the method of claim 9 , wherein said progeny plant has all the morphological and physiological characteristics of the lettuce plant designated 45-140 RZ, representative seed of which having been deposited under NCIMB Accession No. 42471.

15. A method of producing a hybrid lettuce seed comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant hybrid lettuce seed, wherein said first parent lettuce plant or said second parent lettuce plant is the lettuce plant of claim 1 .

16. A hybrid lettuce seed produced by the method of claim 15 .

17. A hybrid lettuce plant grown from the seed as claimed in claim 16 .

18. A method for producing lettuce leaves as a fresh vegetable comprising packaging leaves of a plant of claim 1 .

19. A method for producing lettuce leaves as a processed food comprising processing leaves of a plant of claim 1 .

20. A container comprising one or more lettuce plants of claim 1 for harvest of leaves.

21. A method of determining the genotype of a plant of lettuce variety 45-140 RZ, representative seed of which has been deposited under NCIMB Accession No. 42471, or a first generation progeny thereof, comprising obtaining a sample of nucleic acids from said plant and comparing said nucleic acids to a sample of nucleic acids obtained from a reference plant, and detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms are indicative of lettuce variety 45-140 RZ and/or give rise to the expression of any one or more, or all, of the morphological and physiological characteristics of lettuce variety 45-140 RZ as claimed in claim 1 .

22. The method of claim 21 additionally comprising the step of storing the results of detecting the plurality of polymorphisms on a computer readable medium, or transmitting the results of detecting the plurality of polymorphisms.

23. The lettuce plant of claim 1 , which is a plant grown from seed having been deposited under NCIMB accession No. 42471.

24. A lettuce plant having resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf and other morphological and/or physiological characteristics of a lettuce plant, representative seed of which having been deposited under NCIMB Accession No. 42471.

25. A method of introducing a desired trait into a plant of lettuce variety 45-140 RZ comprising: (a) crossing a plant of lettuce variety 45-140 RZ, representative seed of which having been deposited under NCIMB Accession No. 42471, with a second lettuce plant that comprises the desired trait to produce F1 progeny; (b) selecting an F1 progeny that comprises the desired trait; (c) crossing the selected F1 progeny with a plant of lettuce variety 45-140 RZ, to produce backcross progeny and (d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of a plant of lettuce variety 45-140 RZ, when grown in the same environmental conditions.

26. The method of claim 25 further comprising (e) repeating steps (c) and (d) one or more times in succession to produce selected fourth or higher backcross progeny that comprise the desired trait and all of the physiological and morphological characteristics of a plant of lettuce variety 45-140 RZ, when grown in the same environmental conditions.

27. A lettuce plant produced by the method of claim 25 .

28. A method for producing a seed of a 45-140 RZ-derived lettuce plant comprising (a) crossing a plant of lettuce variety 45-140 RZ, representative seed of which having been deposited under NCIMB Accession No. 42471, with a second lettuce plant, and (b) whereby seed of a 45-140 RZ-derived lettuce plant forms.

29. The method of claim 28 further comprising (c) crossing a plant grown from 45-140 RZ-derived lettuce seed with itself or with a second lettuce plant to yield additional 45-140 RZ-derived lettuce seed, (d) growing the additional 45-140 RZ-derived lettuce seed of step (c) to yield additional 45-140 RZ-derived lettuce plants, and (e) repeating the crossing and growing of steps (c) and (d) for an additional 3-10 generations to generate further 45-140 RZ-derived lettuce plants.

30. The method of claim 29 further comprising selecting at steps b), d), and e), a 45-140 RZ-derived lettuce plant, exhibiting a combination of traits including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf.

31. A seed produced by the method of claim 28 .

32. A method of producing a lettuce cultivar comprising a combination of traits including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf, representative seed of which having been deposited under NCIMB Accession No. 42471, comprising: crossing a mother lettuce plant with a father lettuce plant to produce a hybrid seed; growing said hybrid seed to produce a hybrid plant; selfing said hybrid plant to produce F2 progeny seed; growing said F2 progeny seed to produce F2-plants; selecting said F2-plants for exhibiting including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf, and selfing said selected F2-plants to produce F3 progeny seed; growing said F3 progeny seed to produce F3-plants; selecting F3-plants for exhibiting including resistance to downy mildew ( Bremia lactucae ) races Bl:1 to Bl:32, CA-I to CA-VIII, resistance to currant-lettuce aphid ( Nasonovia ribisnigri ) biotype Nr:0, resistance to root aphids ( Pemphigus bursarius ), and a dull mature leaf, representative seed of which having been deposited under NCIMB Accession No. 42471, optionally followed by more selfing and selection steps.

33. A lettuce cultivar produced by the method of claim 32 .