Probiotic function of human intelectin
The present disclosure provides for methods providing a pre/probiotic agent to a subject. Human Intelectin 1 (hIntL-1) has been shown to bind selectively to glycan components on bacteria, thereby promoting and protecting the microbiome.
1. A method of promoting the growth and/or stability of a microbiome in a subject comprising administering to the subject an intelectin molecule.
2. The method of claim 1 , wherein in intelectin molecule is administered in probiotic formulation containing one or more beneficial microorganisms.
3. The method of claim 2 , wherein said one or more beneficial microorganisms are from the genus Lactobacillus or Bifidobacterium , or a species selected from the group consisting of the bacteria set forth in Table 1.
4. The method of claim 1 , where the intelectin binds to a β-linked D-galactofuranose, a glycan containing a heptose, D-glycero-D-talo-oct-2-ulosonic acid (KO) and/or 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) residue, and/or a saccharide residue modified with a phospho-glycerol (Gro-P) substituent.
5. The method of claim 1 , wherein the intelectin is hIntL-1 or hIntL-2, and the subject is a human, or wherein the intelectin is a non-human intelectin, and the subject is a non-human mammal of the same species as the intelectin.
6. The method of claim 5 , wherein the intelectin is mouse intelectin-1, and the subject is a mouse, or the intelectin is a fish intelectin, and the subject is a fish.
7. The method of claim 1 , wherein the intelectin is PEGylated.
8. The method of claim 1 , wherein the intelectin is administered orally, rectally, vaginally, topically or via inhalation.
9. The method of claim 1 , wherein said subject suffers from one or more of lactose intolerance, antibiotic-induced diarrhea, eczema, Heliobacter pylori infection, irritable bowel syndrome, colitis, necrotizing enterocolitis, bacterial vaginosis, inflammation, high blood pressure, elevated cholesterol, atherosclerosis, obesity, Crohn's Disease, an allergy, asthma, cancer (e.g., colorectal cancer) and/or vitamin deficiency.