Pipetting device, pipette tip coupler, and pipette tip: devices and methods
Pipette assembly comprising a pipette device, a pipette tip, and an expanding mandrel collet coupling device coupling the tip to the pipette device, the coupling device comprising: circumferentially spaced apart segments, an annular base disposed below the segments and comprising a distal portion, an elastomeric element circumscribing the distal portion, and upwardly extending circumferentially spaced apart arms attached at first ends to the annular base and having upper free ends each supporting a different one of the segments configured to expand between a first and a second greater circumference for engaging a first interior working surface of the tip in concurrence with the elastomeric element sealing against a second interior working surface below the first surface and in concurrence with an abutment between a disk exteriorly disposed about a medial portion of the arms and a stepped interior shoulder of the tip disposed between the first and second working surfaces.
1. A pipette tip coupling device for coupling and releasing a pipette tip to and from a pipette device, the pipette tip coupling device comprising:
a central body terminating in a distal end, the central body comprising an interior circumscribing sidewall surface defining an open ended passageway extending through the central body;
an elastomeric element circumscribing the central body adjacent to the distal end of the central body; and
an expanding collet circumscribing the elongated central body comprising:
an annular base, at least a portion of which is proximal to the elastomeric element; and
a segmented collar comprising a plurality of circumferentially spaced apart arm segments with distal ends attached to the annular base and proximal free arm portions, wherein each arm segment comprises an outer surface and an inner surface.
2. The pipette tip coupling device of claim 1 further comprising an annular wedge movably circumscribing the central body, the annular wedge comprising:
a proximal wedge surface;
a distal wedge surface, wherein a circumference of the proximal wedge surface is larger than a circumference of the distal wedge surface;
an exterior surface, wherein the exterior surface connects the proximal wedge surface with the distal wedge surface; and
wherein the exterior surface of the annular wedge abuts against the inner surface of each of the proximal free arm portions of the plurality of the circumferentially spaced apart arm segments.
3. The pipette tip coupling device of claim 1 wherein the segmented collar is capable of radially expanding between a first circumference and a second circumference greater than the first circumference.
4. The pipette tip coupling device of claim 1 wherein each of the plurality of the outer surfaces of the circumferentially spaced apart arm segments further comprises a radially outwardly extending stop disk segment, and wherein the radially outwardly extending stop disk segments form a segmented stop disk.
5. The pipette tip coupling device of claim 2 wherein the segmented collar and the annular wedge are made from an electrically conductive material.
6. A pipette tip coupling device for coupling and releasing a pipette tip to and from a pipette device, the pipette tip coupling device comprising:
a central body terminating in a distal end, the central body comprising an interior circumscribing sidewall surface defining an open ended passageway extending through the central body;
an elastomeric element circumscribing the central body adjacent to the distal end of the central body; and
an expanding collet circumscribing the central body comprising:
an annular base, at least a portion of which is proximal to the elastomeric element, the annular base comprising:
a proximal annular disk; and
a distal reduced diameter annular stem;
a segmented collar comprising a plurality of circumferentially spaced apart arm segments, each of the circumferentially spaced apart arm segments comprising:
an outer surface;
an inner surface;
a proximal free arm portion; and
a distal arm end, wherein the distal arm ends are attached to the proximal annular disk and the proximal free arm portions extend above the annular base.
7. The pipette tip coupling device of claim 6 wherein the elastomeric element comprises an O-ring.
8. The pipette tip coupling device of claim 6 further comprising a spacer, wherein the spacer comprises a cylindrical body circumscribing the central body.
9. The pipette tip coupling device of claim 6 further comprising an annular wedge movably circumscribing the central body, the annular wedge comprising:
a proximal wedge surface;
a distal wedge surface, wherein a circumference of the proximal wedge surface is larger than a circumference of the distal wedge surface;
an exterior surface, wherein the exterior surface connects the proximal wedge surface with the distal wedge surface; and
wherein the exterior surface of the annular wedge abuts against the inner surface of each of the proximal free arm portions of the plurality of the circumferentially spaced apart arm segments.
10. The pipette tip coupling device of claim 6 wherein the segmented collar is capable of radially expanding between a first circumference and a second circumference greater than the first circumference.
11. The pipette tip coupling device of claim 6 wherein each of a plurality of the outer surfaces of the circumferentially spaced apart arm segments further comprises a radially outwardly extending stop disk segment, and wherein the radially outwardly extending stop disk segments form a segmented stop disk.
12. The pipette tip coupling device of claim 9 wherein the segmented collar and the annular wedge are made from an electrically conductive material.
13. A pipette tip coupling device for coupling and releasing a pipette tip to and from a pipette device, the pipette tip coupling device comprising:
a central body comprising:
a proximal shank including a proximal end face;
a distal stem portion;
an end plate distal to the distal stem portion, the end plate having a diameter greater than a diameter of the distal stem portion; and
an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate;
a distal elastomeric element, the distal elastomeric element disposed around the distal stem portion and adjacent to the distal end plate;
an expanding collet disposed around the central body, the expanding collet comprising:
an annular base comprising:
a proximal disk portion; and
a distal collet stem end portion;
a plurality of circumferentially spaced apart, flexible, upwardly extending arms, each circumferentially spaced apart, flexible, upwardly extending arm comprising:
a distal end attached to the disk portion;
a proximal free arm portion; and
an outer surface including a radially outwardly extending stop disk segment; and
wherein the proximal free arm portions of the plurality of circumferentially spaced apart, flexible, upwardly extending arms are capable of expanding radially relative to the central body between an unexpanded state with a first circumference and an expanded state with a second circumference that is larger than the first circumference.
14. The pipette tip coupling device of claim 13 wherein the elastomeric element comprises an O-ring.
15. The pipette tip coupling device of claim 13 ,
wherein the distal stem portion comprises a proximal stem stop shoulder surface having a diameter greater than a diameter of the proximal shank; and
wherein the distal collet stem end portion abuts against the proximal stem stop shoulder surface for securing the collet coaxially with the central body along the longitudinal central axis.
16. The pipette tip coupling device of claim 13 wherein a circumference of the distal elastomeric element is greater than a circumference of the end plate of the central body.
17. The pipette tip coupling device of claim 13 ,
wherein the distal elastomeric element comprises a proximal surface and an interior surface; and
wherein the expanding collet abuts against the proximal surface and the interior surface of the distal elastomeric element.
18. The pipette tip coupling device of claim 13 further comprising an annular wedge movably circumscribing the central body, the annular wedge comprising:
a proximal wedge surface;
a distal wedge surface, wherein a circumference of the proximal wedge surface is larger than a circumference of the distal wedge surface;
an exterior surface, wherein the exterior surface connects the proximal wedge surface with the distal wedge surface; and
wherein the exterior surface of the annular wedge abuts against the inner surface of each of the proximal free arm portions of the plurality of the circumferentially spaced apart arm segments.
19. The pipette tip coupling device of claim 18 wherein the expanding collet and the annular wedge are made from an electrically conductive material.