Methods for fabrication of epinephrine bitartrate nanoparticles and epinephrine bitartrate nanoparticles fabricated thereby
The invention provides a composition including epinephrine nanoparticles and methods for therapeutic use of the composition in the treatment of conditions responsive to epinephrine such as a cardiac event or an allergic reaction, particularly anaphylaxis. The epinephrine nanoparticles can be incorporated into orally-disintegrating and fast-disintegrating tablet pharmaceutical formulations and can significantly increase the sublingual bioavailability of epinephrine, and thereby reduce the epinephrine dose required. Additionally, the invention provides methods for fabrication of stabilized epinephrine nanoparticles for use in the described compositions.
1. A method for fabricating stabilized epinephrine bitartrate nanoparticles, the method comprising:
a) forming a mixture consisting of a pre-determined amount of epinephrine bitartrate and isopropyl alcohol, the pre-determined amount of epinephrine bitartrate combined with the isopropyl alcohol in a reaction chamber; and
b) exposing the mixture formed in step a) to at least a first pass at a pre-determined pressure and a pre-determined temperature, thereby fabricating the stabilized epinephrine bitartrate nanoparticles.
2. The method according to claim 1 , wherein, in the forming step, the pre-determined amount of epinephrine bitartrate has a concentration between about 0.70 mg/ml and about 7.0 mg/ml in the isopropyl alcohol.
3. The method according to claim 1 , wherein the pre-determined pressure for the exposing step is selected in a range from about 8,000 psi to about 30,000 psi and the pre-determined temperature for the exposing step is selected in a range from about 8.3 to about 43.3° C.
4. The method according to claim 1 , further comprising exposing the mixture formed in step a) to multiple passes subsequent to the first pass, the multiple passes at a same or a different pre-determined pressure and at a same or a different pre-determined temperature from that of the first pass.
5. The method according to claim 1 , further comprising lyophilizing the stabilized epinephrine bitartrate nanoparticles fabricated by carrying out steps a)-b).
6. The method according to claim 1 , further comprising measuring particle size of the stabilized epinephrine bitartrate nanoparticles after fabrication, the particle size less than a 1000 nm.