Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions
The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
1. A method of producing a composition for improvement of a tissue in a subject comprising:
culturing fibroblast cells under hypoxic conditions on microcarrier beads or a three dimensional surface in a suitable growth medium, under 1-5% oxygen for at least 2 weeks, thereby producing multipotent stem cells, wherein the multipotent stem cells produce and secrete into the growth medium a composition that promotes repair and regeneration of damaged tissue when administered to the region of tissue in need of repair in the subject, and
collecting the growth medium after the 2 weeks,
thereby producing the composition.
2. The method of claim 1 , wherein the subject has erythema, edema and/or crusting.
3. The method of claim 1 , wherein the cells are cultured on microcarrier beads.
4. The method of claim 1 , wherein the composition comprises a soluble fraction.
5. The method of claim 1 , wherein the composition comprises a non-soluble fraction.
6. The method of claim 1 , wherein the composition comprises a soluble and a non-soluble fraction.
7. The method of claim 1 , wherein the tissue is skin.