Methods and compositions for diagnosis of ectopic pregnancy
Methods and compositions are provided for diagnosing ectopic pregnancy in a mammalian subject by detecting changes in expression of the selected genes, gene fragments or transcripts or expression products, or changes in the expression levels of one or more of proteins or peptide fragments, identified in Table 2 and FIGS. 8 and 9 herein. A selected gene, gene transcript or protein/peptide expression product, or profiles or signatures formed by combinations of same, detected in a biological fluid, preferably sera, of a subject, enables comparison of the corresponding genes, proteins or profiles from that of a reference or control having a normal intrauterine pregnancy. Detection of characteristic changes in the gene profile or protein expression signature of the subject is correlated with a diagnosis of ectopic pregnancy. Various compositions for use in such diagnosis include PCR primer-probe sets or ligands, labeled or immobilized, which are capable of detecting the changes in expression or translation of these targets.
1. A method for diagnosing an ectopic pregnancy in a female mammalian subject comprising:
(a) measuring in a biological fluid sample of the subject the expression levels of the protein or peptides consisting of:
i. Isthmin2 (ISM2),
ii. the pro-domain or extracellular (EC) domain of ADAM12,
iii. pregnancy specific beta-1 glycoprotein isoform 1 (PSG1),
iv. pregnancy specific beta-1 glycoprotein isoform 7 (PSG7),
v. pregnancy specific beta-1 glycoprotein isoform 11 (PSG11),
vi. pregnancy specific beta-1 glycoprotein isoform 9 (PSG9), and
vii. pregnancy specific beta-1 glycoprotein isoform 2 (PSG2);
wherein the expression level of each said protein or peptide is measured by a ligand which binds to the protein or peptide; and
(b) optionally, measuring in said biological fluid sample of the subject the expression level of a protein or peptide selected from the group consisting of:
viii. choriogonadotropin subunit beta precursor (CGB);
ix. glycoprotein hormones alpha chain precursor (CGA);
x. pappalysin-1 precursor (PAPPA);
xi. chorionic somatomammotropin hormone precursor (CSH1); and
xii. progestagen-associated endometrial protein (PAEP);
wherein the expression level of each said selected protein or peptide is measured by a ligand which binds to the protein or peptide; and
(c) comparing said subject's selected protein or peptide expression levels with the levels of the same protein or peptide in the biological fluid of a reference or control female mammalian subject having a normal intrauterine pregnancy (IUP),
wherein changes in expression of the subject's selected proteins or peptides from those of the reference or control correlates with a diagnosis of ectopic pregnancy.
2. The method according to claim 1 , wherein said change in expression level of each said selected protein or peptide comprises an upregulation in comparison to said reference or control or a downregulation in comparison to said reference or control.
3. The method according to claim 1 , wherein the ligand is a protein or peptide.
4. The method according to claim 1 , wherein the ligand is a polynucleotide or oligonucleotide.
5. The method according to claim 1 , wherein the ligand is associated with a detectable label.
6. The method according to claim 1 , wherein said polynucleotide or oligonucleotide is part of a primer-probe set, wherein each primer-probe set amplifies a different gene, or gene expression product.