Automated driving of an assay
The invention herein relates to conducting assays with an apparatus including a substantially transparent assay cartridge loaded with magnetic beads, and a magnet carrier base positioned below a scanning platform holding the assay cartridge. The assay cartridge includes magnetic beads, sample and control solutions in some wells, and assay reagents in others. A microcomputer controls a DC motor which controls movement of the magnet carrier base, and causes the magnetic beads to travel from one well to another. An electromagnetic coil-spring assembly induces mixing of well contents with the magnetic beads on actuation. The assay cartridge is authenticated by sending its encoding to a server or website, and assay instructions are provided remotely to the microcomputer. Following assay completion, the cartridge can have color change or other assay indication detected, and the results sent to the server or website or another recipient.
1. An apparatus for performing an assay where magnetic beads are moved through a plurality of wells which contain assay reagents, comprising:
an assay cartridge having a plurality of wells containing assay reagents, and wherein at least one well contains magnetic beads;
scanning magnets held in a base which is movable with respect to the assay cartridge along a first axis and a second axis, wherein said first axis intersects the second axis, such that movement of the base along the first axis causes the magnetic beads to move from one well to another;
at least two rods extend into the base, the base sliding on the rods to move along the first axis, wherein the rods are in electrical contact with a coil which, upon actuation by a processor configured to selectively apply a potential to the rods from a power source in electrical connection with the rods, oscillates the base along the second axis.
2. The apparatus of claim 1 , wherein the rods are connected with the base with brushes, wires or a combination of the two.
3. The apparatus of claim 1 , wherein the electrical potential of one of the rods is higher than the electrical potential of another rod.
4. The apparatus of claim 1 , wherein the first axis is transverse to the second axis.
5. The apparatus of claim 1 , wherein movement of said base along the first axis is motor-powered by rotation of an externally threaded shaft parallel to the first axis and extending into a matingly threaded portion of the base.
6. The apparatus of claim 1 , wherein the assay cartridge has two parallel rows of wells and the base holds at least two scanning magnets.
7. The apparatus of claim 6 , further including at least two substantially cylindrical orienting magnets, each positioned to magnetically interact with one of the scanning magnets.
8. The apparatus of claim 1 , wherein the power source supplies DC to the rods but is connected with an AC initial source.
9. The apparatus of claim 5 , wherein the processor controls intervals of rotation of the externally threaded shaft.
10. The apparatus of claim 1 , further including a scanner which can scan and record data representing assay results from at least one well of the assay cartridge.
11. The apparatus of claim 10 , further including a transmitter which can transmit said data to a remote location.
12. An apparatus for performing an assay where magnetic beads are moved through a plurality of wells which contain assay reagents, comprising:
an assay cartridge having a plurality of wells containing assay reagents, and wherein at least one well contains magnetic beads;
scanning magnets and orienting magnets positioned in a base and located a specific distance from the assay cartridge;
said base being movable with respect to the assay cartridge along a first axis and a second axis, wherein said first axis intersects the second axis, such that movement of the base along the first axis causes the magnetic beads to move through first channels in the assay cartridge from one well to another;
at least two rods extend into the base, the base sliding on the rods to move along the first axis, wherein the rods are in electrical contact with a coil which, upon actuation, oscillates the base along the second axis using a spring which is compressed upon the coil being actuated; and
a power source and a processor in electrical connection with the rods, the processor configured to apply a potential to the rods which actuates the coil.
13. The apparatus of claim 12 , wherein the first axis is transverse to the second axis.
14. The apparatus of claim 12 , wherein movement of said base along the first axis is powered by motor-driven rotation of an externally threaded shaft parallel to the first axis and extending into a matingly threaded portion of the base.
15. The apparatus of claim 12 , wherein there are two parallel rows of wells and the base holds at least two scanning magnets.
16. The apparatus of claim 12 , wherein the wells are separated by air gaps, formed by second channels extending into the cartridge, and transverse to the first channels.
17. The apparatus of claim 15 , wherein there are at least two substantially cylindrical orienting magnets, wherein the orienting magnets are further from the cartridge than the scanning magnets.
18. The apparatus of claim 14 , further including a microcomputer which controls intervals of rotation of the externally threaded shaft and/or the intervals of potentials provided to the rods.
19. The apparatus of claim 12 , further including a scanner which can scan and record data representing assay results from at least one well of the assay cartridge.
20. The apparatus of claim 19 , further including a transmitter which can transmit said data to a remote location.