IP Library Granted Patent US 10,429,309
Granted Patent B2
US 10,429,309 · App. 15/399,557 · Granted Oct 1, 2019

Apparatus for simultaneous detection of amines and thiols

Inventors: Anthony Burgess-Cassler (Lexington, KY); George David McClure, Jr. (Lexington, KY)
Assignee: ALT BIOSCIENCE, LLC
G01N21/78G01N33/52G01N33/523G01N2021/7759G01N2800/18
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Quick Facts
Patent No.
US 10,429,309
App. No.
15/399,557
Granted
Oct 1, 2019
Kind
B2
Abstract

Disclosed is an apparatus for simultaneous detection of amines and thiols.

Claims (16)

1. An apparatus for the simultaneous detection of amines and thiols, the apparatus comprising a thiol-detection reagent and an amine-detection reagent immobilized onto one or a plurality of pad matrices, wherein the amine-detection reagent comprises phthaldialdehyde (OPA) or fluorescamine, and wherein the one or a plurality of pad matrices is affixed to a plastic backing.

2. An apparatus according to claim 1 , wherein the thiol-detection reagent and the amine-detection reagent are immobilized onto a plurality of pad matrices.

3. An apparatus according to claim 1 , wherein the thiol-detection reagent and the amine-detection reagent are immobilized onto one pad matrix.

4. An apparatus according claim 1 , wherein the thiol-detection reagent comprises DTNB.

5. An apparatus according to claim 1 , for direct in situ contact with a fluid sample.

6. An apparatus according to claim 5 , in the form of a test strip.

7. An apparatus according to claim 1 , for contact with a fluid sample that has been collected by a second apparatus.

8. An apparatus according to claim 7 , in the form of a test strip.

9. An apparatus according to claim 7 , wherein the thiol-detection reagent and the amine-detection reagent are immobilized onto a plurality of pad matrices.

10. An apparatus according to claim 9 , wherein the thiol-detection reagent is immobilized onto a first pad matrix and the amine-detection reagent is immobilized onto a second pad matrix, and wherein the first pad matrix and the second pad matrix may be simultaneously contacted with the second apparatus.

11. An apparatus according to claim 10 , wherein the first pad matrix and the second pad matrix may be simultaneously contacted with the second apparatus through opposition.

12. An apparatus according to claim 1 , for the detection of halitosis.

13. A process for detection and/or monitoring of a physiological, pathophysiological and/or environmental condition characterized by a fluctuation in concentration of amines and/or thiols, the process comprising obtaining a sample from a locus believed to be representative of the physiological, pathophysiological and/or environmental condition and contacting the sample with an apparatus for the detection of amines and thiols, the apparatus comprising a thiol-detection reagent and an amine-detection reagent immobilized onto one or a plurality of pad matrices, wherein the amine-detection reagent comprises phthaldialdehyde (OPA) or fluorescamine.

14. A process according to claim 13 , wherein the obtaining is performed by collection onto an apparatus that is distinct from the apparatus comprising the thiol-detection reagent and the amine-detection reagent.

15. A process according to claim 13 , wherein the obtaining is performed by collection onto the apparatus comprising the thiol-detection reagent and the amine-detection reagent.

16. A process according to claim 13 , wherein the condition is halitosis.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 7, 2021
From: PERIO HEALTH, LLC
To: PDX BIOTECH LLC
Reel/Frame 058328/0984 →
Continuity (3)
Continuation 14893910
Provisional Application 61956053 · May 30, 2013
Related Publication 20170115229A1 · Apr 27, 2017