Maltosyl-isomaltooligosaccharides
The application describes compositions that include maltosyl-isomalto-oligosaccharides with a desirable mass average molecular weight distribution. In some cases, the compositions can contain at least 3% mannitol.
1. A composition comprising maltosyl-isomaltooligosaccharides with a mass average molecular weight distribution of about 730 to 900 daltons, and where at least 60% of the maltosyl-isomaltooligosaccharides have at least 75% α-(1,6) linkages, and wherein at least 40% of the maltosyl-isomaltooligosaccharides in the composition have a degree of polymerization (DP) of 5 or more.
2. The composition of claim 1 , where the maltosyl-isomaltooligosaccharides have no more than about 18 glucose units.
3. The composition of claim 1 , where the maltosyl-isomaltooligosaccharides have a maltose unit at the reducing end.
4. The composition of claim 1 , with less than 2%/brix isomaltose.
5. The composition of claim 1 , with less than 5%/brix glucose.
6. The composition of claim 1 , with less than 5%/brix sucrose.
7. The composition of claim 1 , with less than 4%/brix fructose.
8. The composition of claim 1 , with less than 7%/brix lactate.
9. The composition of claim 1 , with less than 8%/brix maltose.
10. The composition of claim 1 , with 3% or more than 3%/brix mannitol.
11. The composition of claim 1 , with less than 4%/brix glycerol.
12. The composition of claim 1 , with less than 20%/brix MIMO-DP3.
13. The composition of claim 1 , with less than 30%/brix MIMO-DP4.
14. The composition of claim 1 , with more than 18%/brix MIMO-DP5.
15. The composition of claim 1 , with more than 10%/brix MIMO-DP6.
16. The composition of claim 1 , with more than 1%/brix MIMO-7.
17. The composition of claim 1 , with more than 0.5%/brix MIMO-DP8.
18. The composition of claim 1 , with more than 0.1%/brix MIMO-DP9.
19. The composition of claim 1 , as a concentrated solution.
20. The composition of claim 1 , dried as a powder.
21. The composition of claim 1 , aliquoted into individual servings for human or animal consumption.
22. The composition of claim 1 , aliquoted into individual serving of about 0.25 ml to about 10 ml.
23. A composition generated by a method comprising
(a) contacting a feedstock comprising Leuconostoc citreum ATCC 13146 (NRRL B-742) bacterial cells with a fixed ratio of sucrose to maltose ranging from 2.1 to about 4.5 in an aqueous culture to form a fermentation mixture;
(b) fermenting the fermentation mixture at a pH between 4 and 8;
(c) removing the bacterial cells to generate a cell-free liquor;
(d) polishing the cell-free liquor by removal of insoluble impurities; decolorization; de-ashing); removing protein; removing organic acids; or any combination thereof, to generate a polished product;
e) washing insoluble impurities, decolorization agents, de-ashing agents, evaporating mechanisms, centrifugation pellets, filters, resins, weak base anion resins, chromatographic resins, chromatographic grade gel-type strong acid cation exchange resins, or any combination thereof to generate one or more washes; and combining the one or more washes together, or combining the one or more washes with the cell-free liquor; or combining the one or more washes with the polished product;
wherein the final composition comprises maltosyl-isomaltooligosaccharides with a mass average molecular weight distribution of about 730 to 900 to daltons, where at least 60% of the maltosyl-isomaltooligosaccharides have at least 75% α-(1,6) linkages, and wherein at least 40% of the maltosyl-isomaltooligosaccharides in the composition have a degree of polymerization (DP) of 5 or more.