VACCINE DELIVERY METHOD
The present invention includes a composition including as one component a slurry matrix that is a liquid at room temperature and a gel at physiological pH, physiological salt concentrations and/or physiological temperatures and as a second component one or more antigens. Also include are methods of inducing an immune response in a subject and vaccinating a subject by administering such compositions.
1 . A composition comprising as one component a slurry matrix that is a liquid at room temperature and a gel at physiological pH, physiological salt concentrations and/or physiological temperatures and as another component one or more antigens.
2 . The composition of claim 1 , wherein the slurry matrix comprises a peptide hydrogel, or a derivative thereof.
3 . The composition of claim 1 , wherein the slurry matrix comprises a peptide hydrogel of the peptide scaffold RADARADARADARADA, or a derivative thereof.
4 . The composition of claim 3 , wherein the peptide hydrogel comprises PURAMATRIX, or a derivative thereof.
5 . The composition of claim 1 , wherein the slurry matrix comprises MATRIGEL, or a derivative thereof.
6 . The composition of claim 1 further comprising one of more adjuvants.
7 . The composition of claim 1 , further comprising a Toll-Like Receptor (TLR) agonist and/or a cytokine
8 . The composition of claim 7 , wherein the TLR agonist comprises a TLR4 and/or TLR9 agonist.
9 . The composition of claim 8 , wherein the TLR9 agonist comprises a CpG oligodeoxynucleotide (ODN).
10 . The composition of claim 1 , wherein the antigen comprises a hepatitis antigen, an influenza antigen, a schistosomiasis antigen, and/or a burkolderia antigen, or an antigenic fragment thereof.
11 . A method of producing an immune response in a subject, the method comprising administering a composition of claim 1 to the subject.
12 . A method of delivering one or more immunogenic antigens to a subject, the method comprising administering a composition of claim 1 to the subject.
13 . A method of delivering one or more therapeutic antigens to a subject, the method comprising administering a composition of claim 1 to the subject.
14 . The method of claim 11 , wherein the subject is a domestic livestock or a companion animal.
15 . The method of claim 11 , wherein administration of the composition comprises subcutaneous (sc) delivery, intramuscular (im) delivery, intradermal delivery, transdermal delivery, mucosal delivery, intravaginal delivery, intrarectal delivery, intraperitoneal delivery, inhalation delivery, and/or aerosol delivery.
16 . The method of claim 11 , wherein administration of the composition comprises a primary and/or booster vaccination.
17 . The method of claim 11 , wherein administration of the composition comprises a booster vaccination after a primary vaccination with a polypeptide vaccine or a plasmid DNA vaccine.
18 . A method of producing an anti-schistosome immune response in a bovoid, the method comprising administering a composition comprising as one component a slurry matrix that is a liquid at room temperature and is a gel at physiological conditions and as another component one or more schistosome antigens to the bovoid.
19 . The method of claim 18 , wherein the schistosome antigen comprises a SjCTPI polypeptide, a SjCTPI-Hsp70 polypeptide, a SjC23 polypeptide, and/or a SjC23-Hsp70 polypeptide, or an antigenic fragment thereof.
20 . The method of claim 19 , wherein administration of the composition comprises a booster administered after a primary vaccination with a SjCTPI-Hsp70 plasmid DNA vaccine.