Bioseparation compositions and methods for making and using same
A composition for use in bioseparation. The composition includes a plurality of hollow particles having a siliceous surface. The composition further includes a surface-modifying agent bonded to the hollow particles. The surface-modifying agent includes a binding segment and a reactive segment. The binding segment includes a silyl group and the reactive segment includes a reactive nitrogen group.
1. A method for capturing an analyte, the method comprising:
providing a bioseparation composition comprising:
a plurality of hollow particles; and
a surface-modifying agent bonded to the hollow particles, wherein the surface-modifying agent comprises a binding segment and a reactive segment, wherein the-surface-modifying agent comprises an aminosilane;
wherein the plurality of hollow particles have an average density between about 0.05 and 0.4 g/ml; and
contacting the bioseparation composition with a solution comprising an analyte, wherein the surface-modifying agent captures the analyte by directly coupling the analyte with the aminosilane.
2. The method of claim 1 , further comprising separating the bioseparation composition from the solution.
3. The method of claim 2 , wherein separating the bioseparation composition from the solution comprises allowing the bioseparation composition to float to an upper surface of the solution.
4. The method of claim 1 , wherein the hollow particles comprise glass bubbles.
5. The method of claim 1 , wherein a size distribution of the plurality of hollow particles has a span of less than 1.0.