IP Library › Granted Patent US 12,383,893
Granted Patent B2
US 12,383,893 · App. 18/378,581 · Granted Aug 12, 2025

Method and system for buoyant separation

Inventors: Casey Wegner (Ann Arbor, MI); William Lloyd (Ann Arbor, MI); Brandon H. McNaughton (Ann Arbor, MI); Brittany MacIntyre (Ann Arbor, MI)
Assignee: Akadeum Life Sciences, Inc.
B01L3/50215B04B5/0414B01L2200/0689B01L2300/042B01L2300/0681B01L2400/0633
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Quick Facts
Patent No.
US 12,383,893
App. No.
18/378,581
Granted
Aug 12, 2025
Kind
B2
Abstract

A system for buoyant separation includes a separation container. Additionally or alternatively, the system can include an automated instrument, one or more processing components, and/or any other components. A method for buoyant separation can include any or all of: manipulating the separation container; adding materials to the separation container; removing materials form the separation container; otherwise processing the separation container; and/or any other processes.

Claims (42)

1. A system for a selective containment of a set of materials in a container, the system comprising:

the container defining a cavity;

a drainage hole defined at an inferior region of the container;

a drain stop configurable in a set of operation modes;

a cap removably couplable with a superior region of the container;

a cap insert, the cap insert configured for rotation relative to the cap; and

a venting subsystem comprising:

a first venting aperture defined at a surface of the cap insert;

a second venting aperture defined at a surface of the cap, wherein rotation of the cap relative to the cap insert in a first direction produces:

a switch between operation modes of the set of operation modes of the drain stop;

misalignment of the second venting aperture relative to the first aperture; and

a height gap between the cap and the cap insert, wherein the height gap establishes an air path between the misaligned first and second venting apertures, wherein the container is vented via the air path.

2. The system of claim 1 , wherein the switch between operation modes comprises switching from a closed configuration mode to an open configuration mode, wherein:

in the closed configuration mode, the drain stop prevents passage of materials in the cavity through the drainage hole; and

in the open configuration mode, the drain stop allows passage of materials in the cavity through the drainage hole.

3. The system of claim 1 , wherein at least one of the cap or the cap insert defines a sloped feature, wherein rotation of the cap relative to the cap insert is along the sloped feature.

4. The system of claim 3 , wherein the sloped feature is a ramp.

5. The system of claim 3 , wherein the cap insert defines the sloped feature, wherein rotation of the cap relative to the cap insert in the first direction comprises movement of the cap up the sloped feature.

6. The system of claim 1 , wherein the system is configured for use in a separation of a subset of non-target materials in the set of materials from a subset of target materials in the set of materials, wherein each of the subset of target materials is bound to a buoyant particle, and wherein the switch between operation modes comprises enabling passage of the subset of non-target materials through the drainage hole.

7. The system of claim 1 , wherein the set of operation modes of the drain stop comprises a set of heights of the drain stop relative to the drainage hole.

8. The system of claim 7 , wherein movement of the cap relative to the cap insert in a second direction, the second direction opposing the first direction, decreases a height of the gap.

9. The system of claim 1 , wherein during rotation of the cap relative to the cap insert in the first direction, the first venting aperture is misaligned relative to the second venting aperture.

10. The system of claim 1 , wherein the height gap between the cap and the cap insert comprises a height gap between the surface of the cap and the surface of the cap insert.

11. A system for a selective containment of a set of materials in a container, the system comprising:

the container defining a cavity;

a drainage hole defined at an inferior region of the container;

a drain stop configurable in a set of operation modes;

a cap removably couplable with a superior region of the container;

a rod connected to the drain stop at a first end, and connected to the cap at a second end; and

a cap insert, wherein the rod passes through the cap insert, the cap insert configured for rotation relative to the cap, wherein:

at least one of the cap or the cap insert defines a sloped feature;

rotation of the cap relative to the cap insert in a first direction produces movement along the sloped feature; and

movement along the sloped feature lifts the cap, the rod, and the drain stop relative to the cap insert, producing an increase in height of the drain stop relative to the drainage hole, the increase in height corresponding to a draining operation mode of the drain stop.

12. The system of claim 11 , wherein the set of operation modes of the drain stop are associated with differences in height of the drain stop relative to the drainage hole.

13. The system of claim 11 , wherein the drain stop defines a set of fins, the set of fins configured to wipe an interior surface of the separation container during movement of the cap relative to the cap insert.

14. The system of claim 13 , wherein the system is configured for use in a separation of a subset of non-target materials in the set of materials from a subset of target materials in the set of materials, wherein each of the subset of target materials is bound to a buoyant particle, and wherein the switch between operation modes comprises enabling passage of the subset of non-target materials through the drainage hole.

15. The system of claim 14 , wherein the wiping of the interior surface of the separation container is configured to enable drainage of the subset of non-target materials.

16. The system of claim 11 , wherein the cap insert defines the sloped feature, wherein rotation of the cap relative to the cap insert in the first direction comprises movement of the cap up the sloped feature.

17. The system of claim 16 , wherein the sloped feature is a ramp.

18. The system of claim 17 , wherein the cap defines a protrusion feature at an interior surface of the cap, wherein the protrusion feature moves along the ramp.

19. The system of claim 17 , wherein a height of the ramp is configured to effect a predetermined range of initial drainage flow rates through the drainage hole during an open configuration mode of the set of operation modes.

20. The system of claim 11 , wherein the rod comprises a protrusion, wherein the protrusion limits the increase in height.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 10, 2023
From: WEGNER, CASEY; LLOYD, WILLIAM; MCNAUGHTON, BRANDON H.; MACINTYRE, BRITTANY
To: AKADEUM LIFE SCIENCES, INC.
Reel/Frame 065175/0504 →
Continuity (4)
Continuation 17896800 · Aug 26, 2022
Provisional Application 63346202 · May 26, 2022
Provisional Application 63237498 · Aug 26, 2021
Related Publication 20240066520A1 · Feb 29, 2024
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