Shaped articles including hydrogels and methods of manufacture and use thereof

1. A shaped article comprising a structure and a hydrogel coating thereon, wherein the structure comprises a plurality of members and the hydrogel coating is on each member, wherein the plurality of members are sized and spaced such that each member fits in a separate well in one column or row of a microtiter plate, wherein the hydrogel coating comprises alginic acid conjugated to a polyalkylene oxide and a binding moiety, and wherein the coating on each member does not cover the entire surface of the member.

2. The shaped article of claim 1 , wherein the alginic acid molecules in the hydrogel are crosslinked by a cation.

3. The shaped article of claim 2 , wherein the cation is Li + , Mg 2+ , Ca 2+ , Sr 2+ , Ba 2+ , Zn 2+ , Cu 2+ , or Al 2+ .

4. The shaped article of claim 1 , wherein the polyalkylene oxide is polyethylene oxide.

5. The shaped article of claim 1 , wherein the binding moiety is an antibody or antigen-binding fragment thereof, a peptide, an oligonucleotide, a receptor, or a ligand.

6. The shaped article of claim 5 , wherein the binding moiety is an antibody or antigen-binding fragment thereof.

7. The shaped article of claim 6 , wherein the binding moiety is an anti-CD4 antibody, anti-CD8 antibody, anti-CD15 antibody, anti-CD20 antibody, anti-CD24 antibody, anti-CD30 antibody, anti-CD31 antibody, anti-CD33 antibody, anti-CD34 antibody, anti-CD44 antibody, anti-CD45 antibody, anti-CD52 antibody, anti-CD90 antibody, anti-CD117 antibody, anti-CD133 antibody, anti-CD142 antibody, anti-CD146 antibody, anti-CD184 antibody, anti-CD200 antibody, anti-CD318 antibody, anti-A2B5 antibody, anti-c-Kit antibody, anti-EGFR antibody, anti-FGFR3 antibody, anti-FGFR4 antibody, anti-Flk1 antibody, anti-Frizzled-9 antibody, anti-GLAST antibody, anti-Glut1 antibody, anti-HER2 antibody, anti-α4 integrin antibody, anti-N-CAM antibody, anti-Notch-1 antibody, anti-Notch-2 antibody, anti-Sca1 antibody, anti-SIPRA antibody, anti-somatostatinR1 antibody, anti-somatostatinR2 antibody, anti-somatostatinR3 antibody, anti-somatostatinR4 antibody, anti-somatostatinR5 antibody, anti-SSEA-3 antibody, anti-SSEA-4 antibody, an anti-GPCR antibody, or anti-Stro-1 antibody, or antigen binding fragment thereof.

8. The shaped article of claim 5 , wherein the binding moiety is a peptide.

9. The shaped article of claim 8 , wherein the peptide is TNF-α, IL-1β, IL-2, IL6, IL10, α4-integrin, CD15 or an extracellular fragment thereof, CD20 or an extracellular fragment thereof, CD30 or an extracellular fragment thereof, or VEGF.

10. The shaped article of claim 1 , wherein the structure comprises a polymer, ceramic, glass, or metal.

11. The shaped article of claim 1 , wherein the microtiter plate has 24, 96, 384, or 1536 wells.

12. The shaped article of claim 1 , wherein the structure comprises a tubular portion, and the hydrogel coating is on the interior, exterior, or both of the portion.

13. The shaped article of claim 12 , wherein the structure comprises a capillary tube or a pipette tip.

14. The shaped article of claim 1 , wherein the surface of the structure adjacent to the hydrogel coating is hydrophobic.

15. A method of capturing a target, the method comprising:

(a) providing a shaped article comprising a structure and a hydrogel coating thereon, wherein the structure comprises a plurality of members and the hydrogel coating is on each member, wherein the plurality of members are sized and spaced such that each member fits in a separate well in one column or row of a microtiter plate, and wherein the hydrogel coating comprises alginic acid conjugated to a polyalkylene oxide and a binding moiety;

(b) contacting the hydrogel coating with a first liquid comprising the target under conditions allowing for binding of the target to the binding moiety; and

(c) removing the hydrogel coating from contact with the first liquid, thereby capturing the target.

16. The method of claim 15 , further comprising contacting the hydrogel coating with a second liquid comprising a release agent that releases the target from the shaped article.

17. The method of claim 16 , wherein the release agent is H + or OH − .

18. The method of claim 15 , wherein the alginic acid molecules in the hydrogel are crosslinked by a cation, and the release agent is a chelating agent for the cation.

19. The method of claim 18 , wherein the chelating agent is EDTA, EGTA, sodium citrate, BAPTA, crown ether, cryptand, phenanthroline sulfonate, dipyridyl sulfonate, dioxane, DME, diglyme, or triglyme.

20. The method of claim 15 , wherein step (b) occurs in a well of a microtiter plate.

21. The method of claim 15 , wherein during step (b), the first liquid is agitated.

22. The method of claim 21 , wherein the first composition is agitated by movement of the shaped article.

23. The method of claim 21 , wherein the shaped article comprises a lumen via which the first composition can be drawn in and expelled.

24. A method of producing the shaped article of claim 1 , the method comprising the steps of: (a) contacting the structure with a first liquid comprising alginic acid conjugated to a polyalkylene oxide, so that the first liquid coats the structure, (b) contacting the structure coated with the first liquid with cations to crosslink the alginic acid to form a hydrogel coating on the structure, and (c) conjugating a binding moiety to the hydrogel coating, thereby forming the shaped article.

25. The method of claim 24 , wherein steps (a), (b), and (c) occur in different wells of a microtiter plate.

26. The shaped article of claim 3 , wherein the cation is Ca 2+ .

27. The shaped article of claim 4 , wherein the polyethylene oxide is a branched polyethylene oxide.

28. The shaped article of claim 27 , wherein the polyethylene oxide is a 4-arm branched polyethylene oxide.