Trans-base tunnel reader for sequencing
View Patent ↗The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer.
1. A compound for trapping and reading an analyte in a tunnel junction, wherein the compound is
2. A composition comprising the compound of claim 1 .
3. A molecular recognition device configured to identify at least one portion of a target molecule, the device comprising the compound of claim 1 .
4. The molecular recognition device of claim 3 , further comprising:
a substrate;
a first nanotube passing through at least a portion of the substrate, the first nanotube provided with a nanopore and configured and dimensioned to permit the translocation of a target molecule therethrough;
a second nanotube supported by the substrate and disposed in a fixed relationship with respect to said first nanotube so as to form a first gap with the first nanotube; and
an electrical circuit configured to detect an electrical current between the first and second nanotubes, upon passage of the target molecule past said gap;
wherein the first nanotube comprises a first recognition element connected to an end thereof, the second nanotube comprises a second recognition element connected to a first end thereof, and wherein the first recognition element comprises (6)-mercapto-1H-benzo[d]imidazole-2-carboxamide.
5. The molecular recognition device of claim 4 , wherein the second recognition element comprises (6)-mercapto-1H-benzo[d]imidazole-2-carboxamide.