Point-of-care diagnostic cartridge having a digital micro-fluidic testing substrate
A specimen delivery cartridge includes a lower housing, and an upper housing. The upper housing is coupled to the lower housing at a hinge. The specimen delivery cartridge further comprises a testing chamber comprising a paper testing substrate. The paper testing substrate may include a wicking conduit and a plurality of test areas. The specimen delivery cartridge may also include a lens assembly proximate the plurality of test areas and operable to transmit light emissions from the plurality of test areas to an image sensor of a computing device. In some embodiments, the specimen delivery cartridge includes a testing substrate having a plurality of test areas made of an array of electrodes. Each electrode is printed on a first side of the testing substrate and coupled to a conductive via formed in the testing substrate and a conductive trace printed on a second, opposing side of the testing substrate.
1. A specimen delivery cartridge comprising:
a lower housing;
an upper housing coupled to the lower housing at a hinge;
a specimen collection chamber fluidly coupled to an upstream channel that is operable to receive fluid from the specimen collection chamber;
a splitter comprising a fluid guide that splits the upstream channel into a plurality of downstream wicking channels; and
a testing chamber comprising a paper testing substrate,
wherein the paper testing substrate comprises a plurality of wicking conduits and an array of test areas coupled to the plurality of wicking conduits,
wherein the array of test areas comprising a plurality of rows and a plurality of columns,
wherein each test area in a column of the array is coupled to a common wicking conduit,
wherein each test area in a row of the array is coupled to a distinct wicking conduit, and
wherein the splitter fluidly couples the upstream channel to each of the plurality of wicking conduits and is operable to distribute fluid from the upstream channel to each of the plurality of wicking conduits.
2. The specimen delivery cartridge of claim 1 , further comprising a lens assembly proximate the plurality of test areas and operable to transmit light emissions from the plurality of test areas to an image sensor of a computing device.
3. The specimen delivery cartridge of claim 1 , wherein each wicking conduit comprises a hydrophilic wicking channel coupled to a fluid storage chamber.
4. The specimen delivery cartridge of claim 1 , wherein each of the plurality of test areas comprises a spotted blot reporter.
5. The specimen delivery cartridge of claim 4 , wherein each spotted blot reporter comprises a distinct processing agent that reacts in the presence of a target pathogen.