Selective recovery
Provided herein are methods of selective screening. In addition, various targeting proteins and sequences, as well as methods of their use, are also provided.
1. An AAV vector comprising a capsid protein, said capsid protein comprising at least 6 contiguous amino acids from a sequence selected from the group consisting of TRTNPEA (SEQ ID NO: 56) and SVSKPFL (SEQ ID NO: 28).
2. The AAV vector of claim 1 , wherein the at least 6 contiguous amino acids are inserted between AA588-589 of the capsid protein, said capsid protein is an AAV9 capsid protein comprising SEQ ID NO: 2.
3. The AAV vector of claim 1 , wherein the at least 6 contiguous amino acids are inserted between AA586-592 of the capsid protein, said capsid protein is an AAV9 capsid protein comprising SEQ ID NO: 2.
4. The AAV vector of claim 1 , wherein the capsid protein comprises an amino acid sequence selected from the group consisting of TRTNPEA (SEQ ID NO: 56) and SVSKPFL (SEQ ID NO: 28).
5. The AAV vector of claim 1 , wherein the vector comprises an rAAV genome.
6. The AAV vector of claim 5 , wherein the rAAV genome comprises at least one inverted terminal repeat.
7. An AAV capsid protein comprising at least six contiguous amino acids from a sequence selected from the group consisting of: TRTNPEA (SEQ ID NO: 56) and SVSKPFL (SEQ ID NO: 28).
8. The AAV capsid protein of claim 7 , comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 56 and SEQ ID NO: 28.
9. The AAV capsid protein of claim 7 , further conjugated to a nanoparticle or second molecule.
10. The AAV capsid protein of claim 7 , wherein the AAV is selected from AAV1, AAV2, AAV3, AAV3b, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, human isolate hu.31, human isolate hu.32, rhesus isolate rh.8, and rhesus isolate rh.10.
11. The AAV capsid protein of claim 10 , wherein the AAV is an AAV9.
12. The AAV vector of claim 1 , wherein the capsid protein is part of an AAV9 capsid protein.
13. The AAV vector of claim 12 , wherein the at least 6 contiguous amino acids are inserted into the unstructured surface loop of the AAV9 capsid protein.
14. The AAV vector of claim 12 , wherein the at least 6 contiguous amino acids are inserted between amino acids 586-592 of the AAV9 capsid protein, wherein the AAV capsid protein comprises SEQ ID NO: 2.
15. The AAV vector of claim 1 , wherein the sequence is SVSKPFL (SEQ ID NO: 28).
16. The AAV vector of claim 1 , wherein the sequence is TRTNPEA (SEQ ID NO: 56).
17. The AAV capsid protein of claim 7 , wherein the sequence is SVSKPFL (SEQ ID NO: 28).
18. The AAV capsid protein of claim 7 , wherein the sequence is TRTNPEA (SEQ ID NO: 56).
19. The AAV vector of claim 4 , wherein the sequence is SVSKPFL (SEQ ID NO: 28).
20. The AAV vector of claim 4 , wherein the sequence is TRTNPEA (SEQ ID NO: 56).
21. The AAV capsid protein of claim 8 , wherein the amino acid sequence is SVSKPFL (SEQ ID NO: 28).
22. The AAV capsid protein of claim 8 , wherein the amino acid sequence is TRTNPEA (SEQ ID NO: 56).