Isolation of novel AAV's and uses thereof
The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.
1. An isolated nucleic acid engineered to express an mRNA in a target tissue in a subject, wherein the mRNA contains a 3′ UTR comprising at least one binding site for a miRNA expressed endogenously in an off-target tissue in the subject, and wherein the mRNA is flanked by adeno-associated virus (AAV) inverted terminal repeat sequences (ITRs), wherein the nucleic acid encoding the mRNA is operably linked to a tissue-specific promoter, and wherein transduction of the target tissue with the isolated nucleic acid results in expression of the mRNA in the target tissue for at least three weeks.
2. The isolated nucleic acid of claim 1 , wherein each of the at least one miRNA binding sites targets the same miRNA.
3. The isolated nucleic acid of claim 1 , wherein each of the at least one miRNA binding sites targets a different miRNA.
4. The isolated nucleic acid of claim 1 , wherein the mRNA comprises at least two, or at least three binding sites for the miRNA expressed endogenously in the off-target tissue.
5. The isolated nucleic acid of claim 1 , wherein the target tissue is neuronal, gonad, diaphragm, heart, stomach, liver, muscle, spleen, pancreas, or tumor tissue.
6. The isolated nucleic acid of claim 1 , wherein the mRNA encodes a therapeutic protein, a cancer-related protein, an apoptosis-related protein, or a pro-apoptotic protein.
7. The isolated nucleic acid of claim 1 , wherein the target tissue is neuronal tissue, and wherein the mRNA contains a 3′ UTR comprising one or more binding sites for miR-124.
8. The isolated nucleic acid of claim 1 , wherein, when the miRNA and the RNA transcript are present together in the off-target tissue, the miRNA hybridizes to the at least one binding site of the RNA transcript and inhibits activity of the RNA transcript.
9. A recombinant virus comprising a capsid housing an isolated nucleic acid engineered to express an mRNA in a target tissue in a subject, wherein the mRNA contains a 3′ UTR comprising at least one binding site for a miRNA expressed endogenously in an off-target tissue in the subject, and wherein the mRNA is flanked by adeno-associated virus (AAV) inverted terminal repeat sequences (ITRs), wherein the nucleic acid encoding the mRNA is operably linked to a tissue-specific promoter, and wherein transduction of the target tissue with the isolated nucleic acid results in expression of the mRNA in the target tissue for at least three weeks.
10. The recombinant virus of claim 9 , wherein the target tissue is neuronal tissue.
11. The recombinant virus of claim 9 , wherein the 3′ UTR of the mRNA comprises at least two or at least three binding sites for miR-124.
12. The recombinant virus of claim 9 , wherein, when the miRNA and the RNA transcript are present together in the off-target tissue, the miRNA hybridizes to the at least one binding site of the RNA transcript and inhibits activity of the RNA transcript.
13. A syringe containing an injectable aqueous solution that comprises an isolated nucleic acid engineered to express an mRNA in cells of a target tissue, wherein the mRNA contains a 3′ UTR comprising one or more binding sites for an miRNA, wherein the mRNA is flanked by adeno-associated virus (AAV) inverted terminal repeat sequences (ITRs), wherein the isolated nucleic acid is packaged in an AAV viral capsid, wherein the nucleic acid expressing the mRNA is operably linked to a tissue-specific promoter, and wherein transduction of the target tissue with the isolated nucleic acid results in expression of the mRNA in the target tissue for at least three weeks.
14. The syringe of claim 13 , wherein the target tissue is neuronal tissue.
15. The syringe of claim 13 , wherein the 3′ UTR of the mRNA comprises one or more binding sites for miR-124.
16. A method of delivering a transgene to a subject having a tumor, the method comprising administering by injection into the tumor an aqueous solution that comprises an isolated nucleic acid containing a transgene engineered to express an mRNA in cells of the tumor, wherein the mRNA contains a 3′ UTR comprising one or more binding sites for an miRNA, wherein the transgene is flanked by adeno-associated virus (AAV) inverted terminal repeat sequences (ITRs), and wherein the isolated nucleic acid is packaged in an AAV viral capsid wherein the nucleic acid that expresses the mRNA is operably linked to a tissue-specific promoter, and wherein injection of the aqueous solution with the isolated nucleic acid results in expression of the mRNA in the tumor for at least three weeks.
17. The method of claim 16 , wherein the 3′ UTR of the mRNA comprises one or more binding sites for miR-124.
18. The method of claim 16 , wherein the transgene encodes a gene encoding a therapeutic protein.
19. The isolated nucleic acid of claim 1 , wherein the tissue-specific promoter is a liver-specific thyroxin binding globulin (TBG) promoter, an in insulin promoter, a glucagon promoter, a synapsin-1 (Syn) promoter, a creatine kinase (MCK) promoter, a somatostatin promoter, a pancreatic polypeptide (PPY) promoter, a mammalian desmin (DES) promoter, an α-myosin heavy chain (α-MHC) promoter, a cardiac Troponin T (cTnT) promoter, a β-actin promoter, a hepatitis B virus core promoter, an α-fetoprotein (AFP) promoter, a bone osteocalcin promoter, a CD2 promoter, an immunoglobulin heavy chain promoter, a T cell receptor α-chain promoter, a neuron-specific enolase (NSE) promoter, a neurofilament light-chain gene promoter, or a neuron-specific vgf gene promoter.
20. The recombinant virus of claim 9 , wherein the tissue-specific promoter is a liver-specific thyroxin binding globulin (TBG) promoter, an in insulin promoter, a glucagon promoter, a synapsin-1 (Syn) promoter, a creatine kinase (MCK) promoter, a somatostatin promoter, a pancreatic polypeptide (PPY) promoter, a mammalian desmin (DES) promoter, an α-myosin heavy chain (α-MHC) promoter, a cardiac Troponin T (cTnT) promoter, a β-actin promoter, a hepatitis B virus core promoter, an α-fetoprotein (AFP) promoter, a bone osteocalcin promoter, a CD2 promoter, an immunoglobulin heavy chain promoter, a T cell receptor α-chain promoter, a neuron-specific enolase (NSE) promoter, a neurofilament light-chain gene promoter, or a neuron-specific vgf gene promoter.