System to distinguish between X sperm and Y sperm
Approximations to distinguish between X sperm and Y sperm are obtained by detecting light scattered into a pre-set angular range. The scattered light is scattered in turn by nuclei of sperm moving single-file in a sample container. The sperm are not modified prior to the light scattering and are not modified by the light scattering. Approximations are improved by at least a second detection of scattered light.
1. An apparatus to distinguish an X sperm from a Y sperm, the apparatus comprising:
a first source of first incident light,
the first incident light having a first incident light central ray,
the first incident light having a first incident light wavelength;
a sample container to contain a test sperm from a sample of sperm;
the test sperm and other sperm from the sample of sperm flowing single-file along the sample container,
the test sperm having a nucleus,
the sample container being traversed by the first incident light central ray,
a first scattered light detector,
the first scattered light detector positioned to detect first incident light scattered by the nucleus of the test sperm in the sample container, the detected first incident light seen as first scattered light data (DSL 1 );
the first scattered light data (DSL 1 ) being first incident light scattered by the nucleus of the test sperm into a first preset angular range away from the first incident light central ray,
the first scattered light data (DSL 1 ) comprising a first nucleus scattering peak angle (TP 1 ) away from the first incident light central ray of first incident light scattered by the test sperm nucleus;
the first scattered light detector also detecting first calibration data (CSL 1 ), the first calibration data (CSL 1 ) comprising:
a first approximate Y-nucleus peak angle (CY 1 ) away from the first light central ray of first incident light scattered in-turn by nuclei of Y sperm in the sample of sperm flowing single-file along the sample container,
a first approximate X-nucleus peak angle (CX 1 ) away from the first light central ray of first incident light scattered in-turn by nuclei of X sperm in the sample of sperm flowing single-file along the sample container, and
at least CX 1 and CY 1 being included in the first preset angular range.