Polypeptide inhibitors of Smad3 polypeptide activities
This document provides polypeptide inhibitors of Smad3 polypeptide activities. For example, methods and materials for using polypeptides (e.g., polypeptides designed to include both a cell penetrating amino acid sequence and an amino acid segment of a SH3 domain of a SNX9 polypeptide) to inhibit one or more Smad3 polypeptide activities are provided. This document also provides methods and materials for using RNA interference to treat a disease (e.g., a fibrotic disease) in a mammal (e.g., a human).
1. A polypeptide comprising a cell penetrating amino acid sequence and an amino acid segment of a SH3 domain of a sorting nexin 9 (SNX9) polypeptide, wherein said amino acid segment is less than 45 amino acid residues in length and comprises the amino acid sequence set forth in SEQ ID NO:6 or 7.
2. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.
3. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in any one of SEQ ID NOs:9, 11, 13-16, 18, 19, 21, 22, 24, and 25.
4. The polypeptide of claim 1 , wherein said amino acid segment comprises the amino acid sequence set forth in SEQ ID NO:6.
5. The polypeptide of claim 1 , wherein said amino acid segment comprises the amino acid sequence set forth in SEQ ID NO:7.
6. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:37.
7. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:2.
8. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:3.
9. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:4.
10. The polypeptide of claim 1 , wherein said cell penetrating amino acid sequence comprises the amino acid sequence set forth in SEQ ID NO:5.
11. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:9.
12. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:11.
13. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:13.
14. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:14.
15. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:15.
16. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:16.
17. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:18.
18. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:19.
19. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:21.
20. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:22.
21. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:24.
22. The polypeptide of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:25.