Method for making a biofabricated material containing collagen fibrils
Described herein is a method for producing a biofabricated material from collagen or collagen-like proteins. The collagen or collagen-like proteins are isolated from animal sources or produced by recombinant DNA techniques or by chemical synthesis. The collagen or collagen-like proteins are fibrillated, crosslinked, dehydrated and lubricated thus forming the biofabricated material having a substantially uniform network of collagen fibrils.
1. A method for manufacturing an engineered leather material, the method comprising:
forming an aqueous collagen solution comprising collagen and water;
fibrillating the collagen dissolved in the aqueous collagen solution to form a plurality of collagen fibrils;
adding at least one crosslinking agent to the aqueous solution to form crosslinked collagen fibrils;
dehydrating the crosslinked collagen fibrils to form a sheet of engineered leather material; and
incorporating a lubricant comprising a hydrophobic compound into the sheet of engineered leather material.
2. The method of claim 1 , wherein the collagen comprises non-human collagen.
3. The method of claim 2 , wherein the non-human collagen comprises recombinant non-human collagen, wherein the recombinant non-human collagen contains substantially no hydroxylysine.
4. The method of claim 1 , wherein less than 10% by weight of the collagen fibrils are formed into collagen fibers, wherein each of the collagen fibers has a diameter in a range of 5 μm to 10 μm, or wherein the fibrils are aligned with each other for 100 μm to 400 μm of their lengths, or both.
5. The method of claim 1 , wherein dehydrating the crosslinked collagen fibrils to form the sheet of engineered leather material comprises casting, molding, or forming the crosslinked collagen fibrils into the sheet.
6. The method of claim 1 , wherein fibrillating the collagen comprises adding a salt or a combination of salts to the aqueous collagen solution.
7. The method of claim 1 , wherein fibrillating the collagen comprises adjusting the pH of the aqueous collagen solution to a pH in a range of from 5.5 to 8, and wherein adjusting the pH of the aqueous collagen solution comprises adding an acid, a base, or a buffer to the aqueous collagen solution.
8. The method of claim 1 , wherein the at least one crosslinking agent is selected from the group consisting of an amine, a carboxylic acid, a sulfate, a sulfite, a sulfonate, an aldehyde, a hydrazide, a sulfhydryl, a diazirine, an aryl, an azide, an acrylate, an epoxide, a phenol, a chromium compound, a vegetable tannin, and a syntan.
9. The method of claim 1 , wherein dehydrating the crosslinked collagen fibrils comprises contacting the crosslinked collagen fibrils with at least one dehydrating or dewatering solvent.
10. The method according to claim 1 , wherein the lubricant is uniformly incorporated into the sheet of engineered leather material such that the lubricant has a concentration within a first volume of the sheet that is within 20% of a concentration of the lubricant within a second volume of the sheet.
11. The method of claim 1 , further comprising uniformly applying a surface coating or surface finish on or throughout the crosslinked collagen fibrils, wherein the concentration by weight of the surface coating or surface finish on or in identical unit volumes of the collagen fibrils varies by no more than 20%.
12. The method of claim 1 , further comprising uniformly distributing a dye, a stain, a pigment, a resin, a polymer, or a paint on or throughout the collagen fibrils, wherein the concentration by weight of the dye, the stain, the pigment, the resin, the polymer, or the paint in identical unit volumes of the material varies by no more than 20%.
13. The method of claim 1 , further comprising incorporating into or onto the crosslinked collagen fibrils at least one filler; or further comprising incorporating into or onto the crosslinked collagen fibrils at least one woven or nonwoven material.
14. The method of claim 1 , wherein 5% to 10% by weight of the collagen fibrils are formed into collagen fibers.
15. The method of claim 14 , wherein the collagen fibers have a diameter in a range of from 5 μm to 10 μm.
16. The method of claim 1 , wherein the lubricant is present at a concentration within the sheet of engineered leather material in a range of from 1% by weight to 60% by weight.
17. The method of claim 1 , wherein incorporating the lubricant into the sheet of engineered leather material comprises:
treating the sheet of engineered leather material with a combination of the lubricant and a solvent, and
evaporating the solvent.
18. The method of claim 1 , wherein the hydrophobic compound lubricant comprises a fat, an oil, a polymer, a siloxane, an anionic surfactant, a cationic surfactant, a fatty acid, or a rubber.
19. A method for manufacturing an engineered leather material, the method comprising:
forming an aqueous collagen suspension comprising collagen and water;
fibrillating the collagen suspended in the aqueous collagen suspension to form a plurality of collagen fibrils;
adding at least one crosslinking agent to the aqueous collagen suspension to form crosslinked collagen fibrils;
dehydrating the crosslinked collagen fibrils to form a sheet of engineered leather material; and
incorporating a lubricant comprising a hydrophobic compound into the sheet of engineered leather material.