Methods for preventing or treating disorders by increasing bioavailability of iron and related pharmaceutical formulation
The present disclosure relates to methods of treating, ameliorating or preventing a disorder comprising administering a therapeutically effective amount of a composition to a subject in need thereof, which composition contains a lipocalin mutein or a fragment or a variant thereof capable of increasing the bioavailability of iron in the subject.
1. A pharmaceutical composition comprising a lipocalin mutein or fragment thereof having binding affinity to hepcidin, wherein the lipocalin mutein has the same amino acids as the mutein set forth in SEQ ID NO: 1 at two or more positions corresponding to positions 36, 40, 41, 49, 52, 68, 70, 72, 73, 77, 79, 81, 96, 100, 103, 106, 125, 127, 132, and 134 of the linear polypeptide sequence of mature hNGAL, and wherein the lipocalin mutein or fragment is capable of increasing the bioavailability of iron in a subject in need thereof.
2. The pharmaceutical composition of claim 1 , wherein said composition is adapted for administration via a parenteral or non-parenteral route.
3. The pharmaceutical composition of claim 1 , wherein said composition is adapted for administration via an enteral route.
4. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof is capable of inhibiting binding of hepcidin to a hepcidin specific monoclonal antibody having the variable light chain region shown in SEQ ID No: 6 and the variable heavy chain region shown in SEQ ID No: 7.
5. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof competes for binding to hepcidin with a hepcidin specific monoclonal antibody having the variable light chain region shown in SEQ ID No: 6 and the variable heavy chain region shown in SEQ ID No: 7.
6. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof has the same amino acids as the mutein set forth in SEQ ID NO: 1 at the positions corresponding to positions 36, 40, 41, 49, 52, 68, 70, 72, 73, 77, 79, 81, 96, 100, 103, 106, 125, 127, 132, and 134 of the linear polypeptide sequence of mature hNGAL.
7. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof has at least 75% sequence identity to SEQ ID NO: 1.
8. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof has at least 90% sequence identity to SEQ ID NO: 1.
9. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof has at least 95% sequence identity to SEQ ID NO: 1.
10. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof does not bind to Fe-enterobactin.
11. The pharmaceutical composition of claim 1 , wherein the lipocalin mutein or fragment thereof is conjugated to a compound that extends the serum half-life of the lipocalin mutein or fragment thereof.
12. The pharmaceutical composition of claim 11 , wherein the compound that extends the serum half-life is selected from the group consisting of a polyalkylene glycol molecule, a hydroxyethyl starch, a protein domain, a Fc part of an immunoglobulin, a CH3 domain of an immunoglobulin, a CH4 domain of an immunoglobulin, an albumin-binding peptide, and an albumin-binding protein.
13. The pharmaceutical composition of claim 12 , wherein the lipocalin mutein or fragment thereof is conjugated to a polyalkylene glycol.
14. The pharmaceutical composition of claim 13 , wherein the polyalkylene glycol is polyethylene (PEG) or an activated derivative thereof.
15. The pharmaceutical composition of claim 14 , wherein the polyethylene (PEG) is 30 kilo Daltons in molecular weight.
16. The pharmaceutical composition of claim 15 , wherein the lipocalin mutein or fragment thereof is conjugated to or forms a fusion protein with a moiety selected from the group consisting of an Fc part of an immunoglobulin, a CH3 domain of an immunoglobulin, a CH4 domain of an immunoglobulin, an albumin-binding peptide, and an albumin-binding protein.
17. The pharmaceutical composition of claim 1 , wherein the lipocalin fragment lacks at least one of the N-terminal and/or C-terminal amino acids compared to the linear polypeptide sequence of mature hNGAL.
18. The pharmaceutical composition of claim 1 , wherein the lipocalin fragment lacks at least ten consecutive of the N-terminal and/or C-terminal amino acids compared to the linear polypeptide sequence of mature hNGAL.
19. The pharmaceutical composition of claim 1 , wherein the serum half-life of the lipocalin mutein or fragment thereof is at least 1 day.
20. The pharmaceutical composition of claim 1 , wherein the serum half-life of the lipocalin mutein or fragment thereof is at least 2 days.