Food compositions comprising one or both of recombinant beta-lactoglobulin protein and recombinant alpha-lactalbumin protein
Disclosed herein are methods and compositions including casein, and methods for making these compositions.
1. A food composition comprising:
(i) a recombinant β-lactoglobulin protein and a recombinant α-lactalbumin protein, wherein one or both of the recombinant β-lactoglobulin protein and the recombinant α-lactalbumin protein comprises a sequence that is at least 90% identical to the bovine protein amino acid sequence and has been produced by a fungal cell;
(ii) one or more sweetening agents;
(iii) ash; and
(iv) optionally, one or more lipids,
wherein:
the food composition has one or more characteristics of a dairy food product selected from the group consisting of: taste, aroma, appearance, handling, mouthfeel, density, structure, texture, elasticity, springiness, coagulation, binding, leavening, aeration, foaming, creaminess, and emulsification; and
the food composition does not comprise any other milk proteins than those in (i).
2. The food composition of claim 1 , wherein
the recombinant β-lactoglobulin protein comprises a sequence that is at least 90% identical to the bovine protein amino acid sequence and has been produced by a fungal cell; and
the recombinant α-lactalbumin protein comprises a sequence that is at least 90% identical to the bovine protein amino acid sequence and has been produced by a fungal cell.
3. The food composition of claim 1 , wherein the fungal cell is selected from the group consisting of: a strain of Aspergillus nidulans , a strain of Aspergillus niger , a strain of Aspergillus oryzae , a strain of Candida albicans , a strain of Trichoderma reesei , a strain of Chrysosporium lucknowense , a strain of Fusarium gramineum , a strain of Fusarium venenatum , a strain of Physcomitrella patens , and a strain of Neurospora crassa.
4. The food composition of claim 1 , wherein one or both of the recombinant β-lactoglobulin protein and the recombinant α-lactalbumin protein has been secreted by a fungal cell.
5. The food composition of claim 1 , wherein the food composition does not include an animal-derived compound.
6. The food composition of claim 1 , wherein the food composition does not include any compound isolated from a milk produced by a mammal.
7. The food composition of claim 1 , wherein the food composition does not include at least one compound otherwise present in a mammal-produced milk.
8. The food composition of claim 1 , wherein the food composition comprises a plurality of micelles.
9. The food composition of claim 8 , wherein the plurality of micelles comprises one or both of the recombinant β-lactoglobulin protein and the recombinant α-lactalbumin protein.
10. The food composition of claim 1 , wherein the food composition comprises one or more lipids selected from the group consisting of: plant-derived oils, plant-derived monoglycerides, plant-derived diglycerides, plant-derived triglycerides, plant-derived free fatty acids, and plant-derived phospholipids.
11. The food composition of claim 1 , wherein the one or more sweetening agents are saccharides selected from the group consisting of: glucose, mannose, maltose, fructose, galactose, lactose, sucrose, monatin, and tagatose.
12. The food composition of claim 1 , wherein the one or more sweetening agents are artificial sweeteners selected from the group consisting of: stevia , aspartame, cyclamate, saccharin, sucralose, mogrosides, brazzein, curculin, erythritol, glycyrrhizin, inulin, isomalt, lacititol, mabinlin, malititol, mannitol, miraculin, monatin, monelin, osladin, pentadin, sorbitol, thaumatin, xylitol, acesulfame potassium, advantame, alitame, aspartame-acesulfame, sodium cyclamate, dulcin, glucin, neohesperidin dihydrochalcone, neotame, and P-4000.
13. The food composition of claim 1 , wherein the ash comprises one or more of: calcium, phosphorous, phosphate, potassium, sodium, citrate, sulfate, carbonate, chloride, magnesium, iron, copper, zinc, manganese, selenium, iodine, retinol, carotene, vitamins, vitamin D, vitamin E, vitamin B12, thiamin, and riboflavin, or a salt(s) thereof.
14. The food composition of claim 1 , wherein the ash has a final concentration in the food composition of about 5% w/w to about 7% w/w.
15. The food composition of claim 1 , wherein the food composition is selected from the group consisting of: a yogurt, a cheese, a milk, a butter, a caseinate, a cream, an infant formula, an ice cream, a frozen custard, a cottage cheese, a cream cheese, a crème fraiche, and a curd.
16. The food composition of claim 1 , wherein the food composition is a powder composition.
17. The food composition of claim 15 , wherein the food composition is a yogurt.
18. The food composition of claim 15 , wherein the food composition is a butter.
19. The food composition of claim 15 , wherein the food composition is a caseinate.
20. The food composition of claim 15 , wherein the food composition is a cream.
21. The food composition of claim 15 , wherein the food composition is an ice cream.
22. The food composition of claim 15 , wherein the food composition is a frozen custard.
23. The food composition of claim 15 , wherein the food composition is a cottage cheese.
24. The food composition of claim 15 , wherein the food composition is a cream cheese.
25. The food composition of claim 15 , wherein the food composition is a crème fraiche.
26. The food composition of claim 15 , wherein the food composition is a curd.
27. The food composition of claim 7 , wherein the food composition does not include lactose.
28. The food composition of claim 7 , wherein the food composition does not include a hormone.
29. The food composition of claim 15 , wherein the food composition is a milk.
30. The food composition of claim 15 , wherein the food composition is a cheese.