Method for treating diabetes and other glucose regulation disorders using stem cells
Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.
1. A method for treating diabetes, the method comprising:
obtaining gastrointestinal hormone producing cells from a donor; wherein said gastrointestinal hormone regulates glucose in the body of a human;
obtaining stem cells from a patient;
culturing the gastrointestinal hormone producing cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived hormone producing-cells (SCDHPC); and
introducing an amount of the SCDHPC into the patient sufficient to cause a lowering of the patient's blood glucose level after the ingestion of food,
wherein the gastrointestinal hormone producing cells are incretin-producing cells and wherein the gastrointestinal hormone is an incretin.
2. The method of claim 1 , wherein the gastrointestinal hormone producing cells are L-cells or K-cells.
3. The method of claim 1 , wherein the method is used for treating Type I diabetes.
4. The method of claim 1 , wherein the method is used for treating Type II diabetes.
5. The method of claim 1 , wherein the stem cells are induced pluripotent stem cells.
6. The method of claim 1 , wherein the stem cells are pluripotent stem cells.
7. The method of claim 1 , wherein the stem cells are multipotent stem cells.
8. The method of claim 1 , wherein the stem cells are mesenchymal stem cells.
9. The method of claim 1 , wherein the stem cells are oligopotent stem cells.
10. The method of claim 1 , wherein the SCDHPC do not express a pluripotent stem cell marker.
11. The method of claim 10 , wherein the SCDHPC do not express the cell surface antigen SSEA3.
12. The method of claim 10 , wherein the SCDHPC do not express the cell surface antigen SSEA4.
13. The method of claim 10 , wherein the SCDHPC do not express the cell surface antigen Tra-1-60.
14. The method of claim 10 wherein the SCDHPC do not express the cell surface antigen Tra-1-81.
15. The method of claim 1 , wherein the SCDHPC do not express a multipotent stem cell marker.
16. The method of claim 1 , wherein the SCDHPC do not express an oligopotent stem cell marker.
17. The method of claim 1 , wherein the SCDHPC express at least one cell surface antigen mimic of a cell surface antigen of the gastrointestinal hormone producing cells obtained from the donor.
18. The method of claim 1 , wherein the patient's glucose level is lowered below about 150 mg/dl within two hours after ingestion of food.
19. The method of claim 1 , wherein the food comprises a carbohydrate or a fat.
20. A method for treating diabetes, the method comprising:
obtaining gastrointestinal hormone producing cells from a donor; wherein said gastrointestinal hormone regulates glucose in the body of a human;
obtaining stem cells from a patient;
culturing the gastrointestinal hormone producing cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived hormone producing-cells (SCDHPC); and
introducing an amount of the SCDHPC into the patient sufficient to maintain the patient's blood glucose levels within euglycemic levels for an extended period,
wherein the gastrointestinal hormone producing cells are incretin-producing cells and wherein the gastrointestinal hormone is an incretin.
21. The method of claim 20 , wherein the gastrointestinal hormone producing cells are L-cells or K-cells.