Compositions and methods for reducing prion levels
Compositions and methods for reducing the level of prions in a prion-infected cells or host by exposing prion infected cells, tissues and organs to AR-12 and the AR-12 analog AR-14 to reduce the prion level by at least about 90%.
1. A method of reducing the level of prions in prion-infected cells, tissues or organs, comprising exposing prion-infected cells, tissues or organs to AR-12 in an amount sufficient to reduce the level of prions in the prion-infected cells, tissues or organs by at least about 50% compared to prion-infected cells, tissues, organs that have not been exposed to AR-12.
2. The method of claim 1 , wherein the prion level is reduced by at least about 90%.
3. The method of claim 2 , wherein prion infected cells, tissues or organs are exposed to a concentration of AR-12 of at least about 1 μM.
4. The method of claim 3 , wherein prion infected cells, tissues or organs are exposed to a concentration of AR-12 between about 1 μM and 3 μM.
5. The method of claim 1 , wherein the amount of AR-12 the prion infected cells, tissues, and organs are exposed to is sufficient to reduce the prion level by at least about 50% to about 100%.
6. The method of claim 5 , wherein the amount of AR-12 is non-toxic.
7. A method of reducing the level of prions in prion-infected cells, tissues or organs, comprising exposing prion-infected cells, tissues or organs to AR-14 in amount sufficient to reduce the level of prions in the prion-infected cells, tissues or organs by at least about 50% compared to prion-infected cells, tissues, organs that have not been exposed to AR-14.
8. The method of claim 7 , wherein prion infected cell, or organs are exposed to a concentration of AR-14 of at least about 1 μM.
9. The method of claim 8 , wherein prion infected cells, tissues or organs are exposed to a concentration of AR-14 between about 0.5 μM and 2 μM.
10. The method of claim 8 , wherein prion infected cells, tissues or organs are exposed to AR-14 in an amount sufficient to achieve a concentration of AR-14 between about 0.5 μM and 2 μM in the prion infected cells, tissues, or organs.
11. The method of claim 7 , wherein the amount of AR-14 the prion infected cells, tissues, and organs are exposed to is sufficient to reduce the prion level by at least about 50% to about 100%.
12. The method of claim 11 , wherein the amount of AR-14 is non-toxic.