Use of a tetraphenylborate (TPB) salt for the separation of biomolecules
Process for the separation of a biomolecule containing at least one cationic group from a liquid medium containing the biomolecule, which includes the use of a tetraphenylborate (TPB) salt.
1. A process for the separation of a biomolecule from a liquid medium, wherein the biomolecule:
i) is selected from the group consisting of a peptide, a peptide derivative, and combinations thereof; and
ii) comprises between 1 and 20 cationic group(s), wherein at least one cationic group is a guanidine group;
and wherein the process comprises the steps of:
a) providing a first organic solution containing the biomolecule; and
b) adding a tetraphenylborate (TPB) salt together with an alkaline agent to the first organic solution to form a biomolecule-TPB salt in the first organic solution,
c) concentrating the first organic solution containing the biomolecule-TPB salt,
wherein the process further comprises the step of performing a synthesis step without isolation of the biomolecule-TPB salt, wherein said synthesis step comprises coupling, deprotection, and purification.
2. The process of claim 1 comprising the preliminary step of washing the first organic solution containing the biomolecule with an aqueous solution before the addition of the tetraphenylborate salt.
3. The process of claim 1 , further comprising the step of washing the first organic solution or the concentrated organic solution containing the biomolecule-TPB salt with an aqueous solution after the addition of tetraphenylborate salt.
4. The process of claim 1 , wherein the tetraphenylborate salt is NaTPB.
5. The process of claim 1 , wherein the at least one cationic group is an amino group or a guanidine group.
6. The process of claim 1 , wherein the alkaline agent is an inorganic base.
7. The process of claim 1 , wherein the first organic solution comprises an organic solvent is selected from the group consisting of halocarbons, esters, ethers and combinations thereof.
8. A process for the separation of a biomolecule from a liquid medium, wherein the biomolecule:
i) is selected from the group consisting of a peptide, a peptide derivative, and combinations thereof; and
ii) comprises between 1 and 20 cationic group(s), wherein at least one cationic group is a guanidine group;
and wherein the process comprises the steps of:
a) providing a first organic solution containing the biomolecule; and
b) adding a tetraphenylborate (TPB) salt together with an alkaline agent to the first organic solution to form a biomolecule-TPB salt in the first organic solution,
c) concentrating the first organic solution containing the biomolecule-TPB salt; adding to the concentrated organic solution containing the biomolecule-TPB salt an organic solvent selected from the group consisting of alcohols, amide-type solvents, halocarbons, esters, ethers, and combinations thereof, to form a second organic solution containing the biomolecule-TPB salt,
wherein said process further comprises the step of performing a synthesis step without isolation of the biomolecule-TPB salt, wherein the synthesis step comprises coupling, deprotection, and purification.
9. The process of claim 8 , wherein the alkaline agent is an inorganic base.
10. The process of claim 8 , wherein the first organic solution comprises an organic solvent selected from the group consisting of halocarbons, esters, ethers and combinations thereof.
11. The process according to claim 8 , wherein the biomolecule is precipitated or crystallized as a solid biomolecule-TPB salt from the second organic solution.