Hyper-productive chromatography system and process

1. A method of performing a Hyper-Productive chromatography process comprising:

providing a stackable chromatographic cassette;

loading a sample to be processed;

operating the stackable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr;

and

wherein a residence time of the loading step is less than one minute.

2. The method of claim 1 wherein the adsorptive chromatographic bed comprises one of:

a polymeric media;

a hydrogel media;

a compressible adsorptive media; and

a semi-compressible adsorptive media.

3. The method of claim 2 wherein the polymeric media is polymethylmethacrylate (PMMA).

4. The method of claim 1 wherein the linear velocity is greater than 1000 cm/hr.

5. The method of claim 1 wherein the residence time in the loading step is less than 0.5 minute.

6. The method of claim 1 wherein the residence time in the loading step is less than 0.25 minute.

7. A method of performing a Hyper-Productive chromatography process comprising:

providing at least one chromatographic cassette having an adsorptive chromatographic bed having a bed volume greater than one liter and comprising one of:

compressible chromatographic adsorptive media;

semi-compressible chromatographic adsorptive media;

soft chromatographic adsorptive media;

placing the at least one chromatographic cassette in a cassette holder;

loading a sample to be processed;

operating the chromatographic adsorptive bed establishing a flow at a velocity greater than 1000 cm/hr; and

wherein a residence time in the loading step is less than one minute.

8. The method of claim 7 wherein the residence time in the loading step is less than 0.5 minutes.

9. The method of claim 7 wherein the residence time in the loading step is less than 0.25 minutes.

10. The method of claim 7 wherein soft chromatographic media comprises one of:

polymethylmethacrylate (PMMA);

agarose Protein-A media;

cation exchange media;

anion exchange media;

mixed-mode media;

size-exclusion chromatography (“SEC”) media;

controlled-pore glass (“CPG”) media.

11. The method of claim 7 further comprising operating the chromatographic cassette having a linear pressure drop over a velocity range of zero cm/hr to about 1200 cm/hr.

12. The method of claim 7 , further comprising operating at a total cycle productivity of greater than about 60 g/L/hr (GLH), with media having a average bead size less than about 50 μm at velocities greater than about 500 cm/hr.

13. The method of claim 7 , further comprising operating at a total cycle productivity of greater than 100 g/L/hr (GLH) with media having a average bead size less than about 40 μm at velocities greater than about 800 cm/hr.

14. The method of claim 7 , further comprising operating at a total cycle productivity of greater than 150 g/L/hr (GLH) with media having a average bead size less than about 30 μm at velocities greater than about 1000 cm/hr.

15. The method of claim 7 , wherein the adsorptive chromatographic bed comprises chromatographic beads having an average bead size less than 30 μm.

16. The method of claim 7 further comprising stacking a plurality of chromatographic cassettes within the cassette holder to form a chromatographic adsorptive bed having a scaled up bed volume larger than about five liters.

17. The method of claim 7 further comprising operating at a constant hydraulic resistance independent of velocity.

18. A system for performing a Hyper-Productive chromatography process comprising:

a scalable chromatographic cassette holder;

wherein the stackable chromatographic cassette is disposed in the scalable chromatographic cassette holder; wherein the stackable chromatographic cassette is capable of being operated according to claim 1 .

19. The system of claim 18 , wherein the stackable chromatographic cassette comprises an fused deposition modeling (FDM) 3-D printed lattice having porous printed surfaces sealed with a sealant.

20. The system of claim 19 , wherein the sealant is one of:

epoxy;

polyurethane;

silicone; and

UV-curable adhesive.

21. The system of claim 19 further comprising a chamber enclosing the lattice; and

wherein the sealant is applied to one of:

an outside surface of the chamber enclosing the lattice; and

an inside surface of the chamber enclosing the lattice, where the sealant impregnates wetted surfaces of the chamber and excess sealant is withdrawn before the excess sealant cures.