Human soluble CD146, preparation and uses thereof
The present invention relates to compositions and methods for modulating angiogenesis in vivo, ex vivo or in vitro. More particularly, the invention relates to a soluble CD146 protein usable in the context of human therapy, as well as to corresponding antibodies. Particular forms of CD146, herein described, may be used to mobilize, in vivo or ex vivo, both mature and immature endothelial cells, as well as to increase their influence on angiogenesis. The invention also relates to compositions comprising such compounds, particularly pharmaceutical or diagnostic compositions, including kits and the like, as well as methods of therapy or diagnosis using said compounds, compositions and cells.
1. A method of treating a soluble CD146 (sCD146) expressing cancer in a mammal comprising administering a monoclonal antibody that specifically binds to a human soluble CD146 protein selected from SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 7, or a composition comprising said antibody and a pharmaceutically acceptable carrier, to a mammal having a sCD146 expressing cancer, wherein:
the monoclonal antibody comprises a heavy chain comprising SEQ ID NO: 27 (CDR1), SEQ ID NO: 28 (CDR2) and SEQ ID NO: 29 (CDR3) and a light chain comprising SEQ ID NO: 30 (CDR1), SEQ ID NO: 31 (CDR2) and SEQ ID NO: 32 (CDR3).
2. The method according to claim 1 , wherein the cancer is a breast cancer or a melanoma.
3. The method according to claim 2 , wherein the cancer is a breast cancer.
4. The method according to claim 2 , wherein the cancer is a melanoma.
5. The method according to claim 1 , wherein the mammal is a human.
6. The method according to claim 1 , wherein the monoclonal antibody is produced by the hybridoma 2B9-4, deposited as CNCM 1-5507.