METHODS FOR CULTURING AND ANALYZING CELLS
A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.
1 . A system for analyzing a biological sample, the system comprising:
at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source;
a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device;
a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and
an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.
2 . The system according to claim 1 , wherein the droplet generator device is a liquid bridge.
3 . The system according to claim 1 , further comprising a thermocycler.
4 . The system according to claim 1 , wherein the sample droplets are dispensed into separate wells of a well plate.
5 . The system according to claim 1 , wherein the system is configured to dispense the sample droplets as discrete spots on a surface to form an array of droplets.