Detection of a posttranslationally modified polypeptide by a bivalent binding agent
A bivalent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bivalent binding agent, and for use of the disclosed bivalent binding agent in histological staining procedures.
1. A bivalent binding agent capable of binding at least a first epitope and a second epitope of phosphorylated insulin-like growth factor-1 receptor (pIGF-1R) wherein the bivalent binding agent consists of: a first monovalent binder that specifically binds to a polypeptide epitope of SEQ ID NO:11, wherein the first monovalent binder consists of: a Fab′-fragment of mAb 1.4.168 and a first ssDNA of SEQ ID NO:6, the first monovalent binder having a dissociation constant (Kdiss) ranging from 5×10 −3 /sec to 1×10 −4 /sec; a second monovalent binder that specifically binds to a posttranslational polypeptide modification of SEQ ID NO:11 corresponding to phosphorylation of tyrosine residue 1346, wherein the second monovalent binder consists of: a Fab′-fragment selected from the group consisting of mAb 8.1.2 and mAb 30.4.33 and a second ssDNA of SEQ ID NO:5, the second monovalent binder having a Kdiss ranging from 5×10 −3 /sec to 1×10 −4 /sec; and a linker selected from the group consisting of SEQ ID NO:27, SEQ ID NO:28, and SEQ ID NO:29, linking the first monovalent binder to the second monovalent binder, the bivalent binding agent having a Kdiss of 3×10 −5 /sec or less.
2. The bivalent binding agent of claim 1 , wherein the linker has a length of 6 to 100 nm.
3. The bivalent binding agent of claim 1 , wherein the linker is an L-DNA-linker.