Methods of treating burns with i.v. cP12 in a window from 2 to 6 hours after injury
A treatment window for the intravenous treatment of wounds, including thermal and chemical burns, with cP12 is presented. In particular, Applicants have unexpectedly found that delaying intravenous treatment with fibronectin-derived peptides, such as cP12, from 2 to 6 hours, particularly about 4 hours, after wounding, provides superior wound-closing results than treatment at 1 hour or after 8 or more hours.
1. A method of treating a patient with a wound selected from the group consisting of a surgical incision or extirpation, a traumatic injury, a thermal burn, a chemical burn, a lesion or ulceration of the patient's skin, mucosa, connective tissue, fascia, ligament, tendon, cartilage, nerve, or muscle, and a wound to the patient's bone, the method comprising:
intravenously administering to the patient a therapeutically effective amount of a cyclized form of a polypeptide consisting of amino acids PSHISKYILRWRPK (SEQ ID NO:2), wherein the dosage is from 0.003 to 0.1 mg/kg and wherein the intravenous administration occurs from about 2 hours to about 6 hours after formation of the wound.
2. The method of claim 1 , wherein the wound is a thermal burn or a chemical burn.
3. The method of claim 2 , wherein the wound is a thermal burn.
4. The method of claim 2 , wherein the intravenous administration is initiated about 4 hours after formation of the wound and the intravenous administration is given over about a half-hour period.
5. The method of claim 4 , wherein the dosage is from 0.03 to 0.1 mg/kg.
6. The method of claim 3 , wherein the intravenous administration is initiated about 4 hours after formation of the wound and the intravenous administration is given over about a half-hour period.
7. The method of claim 6 , wherein the dosage is from 0.03 to 0.1 mg/kg.
8. The method of claim 1 , wherein the polypeptide is cyclized by chemical crosslinking, chemical intramolecular ligation or enzymatic intramolecular ligation.