Apparatus and method for extracting pathogens from biological samples
The disclosed embodiments related to an apparatus and methods for biological sample processing enabling isolation and concentration of microbial or pathogenic constituents from the sample. Sample may be obtained directly from a specimen container, such as a vacutainer, and processed directly without risk of user exposure. The disclosed methods and apparatus provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.
1. An apparatus to capture pathogens from a biological sample, the apparatus comprising:
a valve body to fluidically couple a sample tube to each of a syringe body and a capture tube, the valve body having a sample inlet, a first outlet, a second outlet, a channel to connect the sample inlet with each of the first and the second outlets, a first one-way valve to connect the sample inlet with the channel, a second one-way valve to connect the channel to the second outlet;
a syringe body having an outer barrel and an inner barrel, the outer barrel further comprising a first reservoir containing a first reagent; and
a plunger piston configured to moveably couple to the outer syringe barrel, the plunger piston comprising an inner syringe barrel, and outer syringe barrel and a puncture-able separator positioned between the first and second reservoirs, the inner syringe barrel defining a second reservoir to receive a second reagent;
wherein the puncture-able separator is configured to provide automatic sequential delivery of the first and second reagents.
2. The apparatus of claim 1 , wherein the valve body is configured to receive a sample tube and fluidically connect the sample to the inlet channel.
3. The apparatus of claim 2 , wherein the valve body further comprises a piercing member configured to pierce the puncture-able separator to thereby access the second reagent.
4. The apparatus of claim 3 , wherein the piercing member is configured to enter the second reservoir when the outer syringe, containing the first reagent, is nearly fully displaced, and the first reservoir is substantially empty.
5. The apparatus of claim 4 , wherein the puncture-able separator which separates the first and the second reservoirs is an elastomer diaphragm.
6. The apparatus of claim 2 , wherein the valve body further comprises at least one inlet piercing member, the inlet piercing member configured to couple through the one way valve to the sample tube.
7. The apparatus of claim 2 , wherein the valve body further comprises a first and a second piercing members configured to enter the sample tube, the first piercing member to fluidically couple to the channel, the second piercing member to fluidically communicate with the ambient.
8. The apparatus of claim 7 , wherein the second piercing member further comprises a one-way valve to allow ingress of ambient air into the sample tube.
9. The apparatus of claim 1 , further comprising a capture tube to removably connect to the valve body, the capture tube having at least one filter membrane configured such that all fluids exiting the valve body pass through the at least one membrane.
10. The apparatus of claim 1 , wherein the first one-way valve opens under a relative negative pressure on the syringe side of the valve and closes under a relative positive pressure on the syringe side of the valve.
11. The apparatus of claim 1 , wherein the second one-way valve opens under a relative positive pressure on the syringe side of the valve, and closes under a relative negative pressure on the syringe side of the valve.
12. An apparatus to mix a sample with a pre-defined reagent, the apparatus comprising:
a syringe body having an outer barrel and an inner barrel;
a plunger piston configured to moveably couple to the syringe body, the plunger piston having a reservoir and a plunger gasket, the plunger gasket to sealingly couple the plunger piston with the inner barrel of the syringe, the plunger piston further having a reservoir to receive a second reagent; and
a valve body to fluidically couple to the syringe body, the valve body having an inlet, a first outlet, a second outlet, a channel connecting the inlet with the first and the second outlets, a first one-way valve connecting the inlet with the channel and a second one-way valve connecting the channel to the second outlet.
13. The apparatus of claim 12 , wherein the outer barrel mates with the valve body through syringe coupler 115 .
14. The apparatus of claim 12 , wherein the valve body is configured to receive a sample tube and connect the sample tube to the inlet.
15. The apparatus of claim 14 , wherein the valve body further comprises at least one inlet piercing member, the at least one inlet piercing member configured to couple the valve channel with the sample tube.
16. The apparatus of claim 12 , wherein the plunger further comprises an auxiliary reservoir to receive a second processing fluid.
17. The apparatus of claim 16 , wherein the valve body further comprises a piercing member to pierce the plunger gasket to thereby access the second reagent.
18. The apparatus of claim 16 , wherein the piercing member is configured to extends to the auxiliary reservoir.
19. The apparatus of claim 16 , wherein the plunger reservoir and the plunger auxiliary reservoir are separated by a diaphragm.
20. The apparatus of claim 12 , further comprising a capture tube to connect the valve body to couple to the valve body, the capture tube having a filter membrane to capture at least one effluent from the second outlet of the valve body.
21. The apparatus of claim 12 , wherein the first one-way valve opens under a relative negative pressure and closes under a relative positive pressure.
22. The apparatus of claim 12 , wherein the second one-way valve closes under a relative negative pressure and closes under a relative positive pressure.