Phage derived antimicrobial activities
The present invention provides methods and compositions to reduce growth of microbial colonies, including infections, and includes therapeutic compositions, methods for treatment of infections, and methods for identifying additional such compositions.
1. An expression vector comprising an isolated or recombinant nucleic acid encoding a chimeric polypeptide, wherein the chimeric polypeptide comprises a muralytic domain (MD) having at least 90% sequence identity to the sequence of amino acids 669-808 of SEQ ID NO: 1 and a heterologous cell binding domain (CBD) that binds to a target bacterium, wherein the CBD has a sequence with at least 90% identity to a sequence selected from the group consisting of:
amino acids 144-240 of SEQ ID NO:4,
amino acids 149-257 of SEQ ID NO:8, and
amino acids 144-243 of SEQ ID NO:12,
wherein the target bacterium exhibits reduced or no growth after being contacted with the chimeric polypeptide.
2. The expression vector of claim 1 , wherein the chimeric polypeptide comprises a sequence with at least 90% identity to SEQ ID NO:4.
3. A cell comprising the nucleic acid of claim 1 .
4. The cell of claim 3 , which is a prokaryotic cell.
5. The cell of claim 3 , which secretes the chimeric polypeptide.