Integrated heater and magnetic separator
An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.
1. A method of extracting nucleic acids using a rack and an integrated separator and heater, the integrated separator and heater comprising a plurality of heater units aligned collinearly along a horizontal first axis, the integrated separator and heater further comprising one or more magnets disposed on a first side of the plurality of heater units and aligned along a second axis parallel to the first axis, the method comprising:
inserting a plurality of holders in the rack, each holder comprising a single process tube of a plurality of process tubes;
inserting the rack into the integrated separator and heater such that at least a portion of rack is removably received on a second side of the plurality of heater units opposite the one or more magnets on the first side, wherein a portion of each process tube of the plurality of process tubes is received in a single heater unit of the plurality of heater units when the rack is inserted into the integrated separator and heater;
moving all of the one or more magnets backwards and forwards together in a vertical direction perpendicular to the second axis while maintaining proximity to an exterior side of each of the process tubes received in the integrated separator and heater;
during at least a portion of the motion, moving magnetic particles contained in the process tubes; and
independently controlling each heater unit of the plurality of heater units to apply heat to the plurality of process tubes received in the integrated separator and heater.
2. The method of claim 1 , wherein all of the one or more magnets are moved and the plurality of process tubes are heated at the same time.
3. The method of claim 1 , wherein each heater unit comprises a cavity configured to partially surround a portion of the process tube received in the cavity.
4. The method of claim 3 , wherein the cavity surrounds a lower portion of the process tube on two sides when the rack is inserted into the integrated separator and heater.
5. The method of claim 3 , wherein the cavity surrounds a lower portion of the process tube on three sides when the rack is inserted into the integrated separator and heater.
6. The method of claim 3 , further comprising sensing a temperature of each cavity.
7. The method of claim 1 , wherein the one or more magnets are affixed to a supporting member, and wherein moving all of the one or more magnets comprising moving the supporting member.
8. The method of claim 7 , wherein the supporting member is affixed to a shaft, and wherein moving all of the one or more magnets comprises moving the supporting member along the shaft.
9. The method of claim 8 , further comprising moving the supporting member between a first position situated away from the plurality of process tubes, and a second position situated in close proximity to the plurality of process tubes.
10. The method of claim 9 , further comprising moving the supporting member at an amplitude about the second position where the amplitude is smaller than a distance between the first position and the second position as measured along the shaft.
11. The method of claim 1 , wherein the integrated separator and heater comprises 12 heater units.
12. The method of claim 1 , wherein 12 holders are inserted in the rack.
13. The method of claim 1 , wherein applying heat to the plurality of process tubes received in the integrated separator and heater comprises raising the temperature of a 1 ml sample in one of the process tubes from room temperature to about 65° C. in less than 3 minutes.
14. The method of claim 1 , wherein moving the magnetic particles comprising moving the magnetic particles against a wall of the process tubes.
15. The method of claim 1 , wherein moving the magnetic particles comprising concentrating the magnetic particles in a portion of the process tubes.
16. The method of claim 1 , wherein moving the magnetic particles comprises sliding the magnetic particles toward the bottom of the process tubes.
17. The method of claim 1 , wherein the magnetic particles are in suspension in solution in the process tubes, and wherein moving the magnetic particles comprises collecting the suspended magnetic particles into one location inside the process tubes.
18. The method of claim 17 , further comprising aspirating the solution from the process tubes after collecting the suspended magnetic particles.