Polypeptides for use in self-assembling protein nanostructures
Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.
1. A recombinant nucleic acid encoding a polypeptide comprising an amino acid sequence that is at least 75% identical over its length, and identical at least at one identified interface position, to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-34:
Name
Amino Acid Sequence
Identified interface residues
I53-34A
MEGMDPLAVLAESRLLPLLTVRGGEDLAGLATVLELMGVGALEITL
I53-34A:
SEQ ID
RTEKGLEALKALRKSGLLLGAGTVRSPKEAEAALEAGAAFLVSPGL
28, 32, 36, 37, 186, 188, 191, 192, 195
NO: 1
LEEVAALAQARGVPYLPGVLTPTEVERALALGLSALKFFPAEPFQG
VRVLRAYAEVEPEVRELPTGGIKEEHLPHYAALPNLLAVGGSWLLQ
GDLAAVMKKVKAAKALLSPQAPG
I53-34B
MTKKVGIVDTTFARVDMAEAAIRTLKALSPNIKIIRKTVPGIKDLPV
I53-34B:
SEQ ID
ACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEASLGLMLAQLMT
19, 20, 23, 24, 27, 109, 113, 116, 117,
NO: 2
NKHIIEVFVHEDEAKDDDELDILALVRAIEHAANVYYLLFKPEYLTR
120, 124, 148
MAGKGLRQGREDAGPARE
I53-40A
MTKKVGIVDTTFARVDMASAAILTLKMESPNIKIIRKTVPGIKDLPV
I53-40A:
SEQ ID
ACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEASLGLMLAQLMT
20, 23, 24, 27, 28, 109, 112, 113, 116,
NO: 3
NKHIIEVFVHEDEAKDDAELKILAARRAIEHALNVYYLLFKPEYLTR
120, 124
MAGKGLRQGEEDAGPARE
I53-40B
MSTINNQLKALKVIPVIAIDNAEDIIPLGKVLAENGLPAAEITERSSAA
I53-40B: 47, 51, 54, 58, 74, 102
SEQ ID
VKAIMLLRSAQPEMLIGAGTILNGVQALAAKEAGATFVVSPGFNPN
NO: 4
TVRACQIIGIDIVPGVNNPSTVEAALEMGLTTLKFFPAEASGGISMV
KSLVGPYGDIRLMPTGGITPSNIDNYLAIPQVLACGGTWMVDKKLV
TNGEWDEIARLTREIVEQVNP
I53-47A
MPIFTLNTNIKATDVPSDFLSLTSRLVGLILSKPGSYVAVHINTDQQL
I53-47A: 22, 25, 29, 72, 79, 86, 87
SEQ ID
SFGGSTNPAAFGTLMSIGGIEPSKNRDHSAVLFDHLNAMLGIPKNR
NO: 5
MYIHFVNLNGDDVGWNGTTF
I53-47B
MNQHSHKDYETVRIAVVRARWHADIVDACVEAFEIAMAAIGGDRF
I53-47B:
SEQ ID
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEFV
28, 31, 35, 36, 39, 131, 132, 135, 139,
NO: 6
ASAVIDGMMNVQLSTGVPVLSAVLTPHRYRDSAEHHRFFAAHFAV
146
KGVEAARACIEILAAREKIAA
I53-50A
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVP
I53-50A: 25, 29, 33, 54, 57
SEQ ID
DADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLD
NO: 7
EEISQFCKEKGVFYMPGVMTPTELVKAMKLGHTILKLFPGEVVGPQ
FVKAMKGPFPNVKFVPTGGVNLDNVCEWFKAGVLAVGVGSALVK
GTPDEVREKAKAFVEKIRGCTE
I53-50B
MNQHSHKDYETVRIAVVRARWHAEIVDACVSAFEAAMADIGGDR
I53-50B:
SEQ ID
FAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEF
24, 28, 36, 124, 125, 127, 128, 129,
NO: 8
VASAVIDGMMNVQLSTGVPVLSAVLTPHRYRDSDAHTLLFLALFA
131, 132, 133, 135, 139
VKGMEAARACVEILAAREKIAA
I53-51A
MFTKSGDDGNTNVINKRVGKDSPLVNFLGDLDELNSFIGFAISKIPW
I53-51A:
SEQ ID
EDMKKDLERVQVELFEIGEDLSTQSSKKKIDESYVLWLLAATAIYRI
80, 83, 86, 87, 88, 90, 91, 94, 166, 172,
NO: 9
ESGPVKLFVIPGGSEEASVLHVTRSVARRVERNAVKYTKELPEINR
176
MIIVYLNRLSSLLFAMALVANKRRNQSEKIYEIGKSW
I53-51B
MNQHSHKDYETVRIAVVRARWHADIVDQCVRAFEEAMADAGGDR
I53-51B:
SEQ ID
FAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEF
31, 35, 36, 40, 122, 124, 128, 131, 135,
NO: 10
VASAVIDGMMNVQLSTGVPVLSAVLTPHRYRSSREHHEFFREHFM
139, 143, 146, 147
VKGVEAAAACITILAAREKIAA
I52-03A
MGHTKGPTPQQHDGSALRIGIVHARWNKTIIMPLLIGTIAKLLECGV
I52-03A: 28, 32, 36, 39, 44, 49
SEQ ID
KASNIVVQSVPGSWELPIAVQRLYSASQLQTPSSGPSLSAGDLLGSS
NO: 11
TTDLTALPTTTASSTGPFDALIAIGVLIKGETMHFEYIADSVSHGLMR
VQLDTGVPVIFGVLTVLTDDQAKARAGVIEGSHNHGEDWGLAAVE
MGVRRRDWAAGKTE
I52-03B
MYEVDHADVYDLFYLGRGKDYAAEASDIADLVRSRTPEASSLLDV
I52-03B: 94, 115, 116, 206, 213
SEQ ID
ACGTGTHLEHFTKEFGDTAGLELSEDMLTHARKRLPDATLHQGDM
NO: 12
RDFQLGRKFSAVVSMFSSVGYLKTVAELGAAVASFAEHLEPGGVV
VVEPWWFPETFADGWVSADVVRRDGRTVARVSHSVREGNATRME
VHFTVADPGKGVRHFSDVHLITLFHQREYEAAFMAAGLRVEYLEG
GPSGRGLFVGVPA
I52-32A
MGMKEKEVLIITHGDFGKGLLSGAEVIIGKQENVHTVGLNLGDNIE
I52-32A:
SEQ ID
KVAKEVMRIIIAKLAEDKEIIIVVDLFGGSPFNIALEMMKTEDVKVIT
47, 49, 53, 54, 57, 58, 61, 83, 87, 88
NO: 13
GINMPMLVELLTSINVYDTTELLENISKIGKDGEKVIEKSSLKM
I52-32B
MKYDGSKLRIGILHARWNLEIIAALVAGAIKRLQEFGVKAENIIIETV
I52-32B: 19, 20, 23, 30, 40
SEQ ID
PGSFELPYGSKLFVEKQKRLGKPLDAIIPIGVLEKGSTMHFEYICDSTT
NO: 14
HQLMKLNFELGIPVIFGVLTCLTDEQAEARAGLIEGKMHNHGEDW
GAAAVEMATKFN
I52-33A
MAVKGLGEVDQKYDGSKLRIGILHARWNRKIILALVAGAVLRLLEF
I52-33 A: 33, 41, 44, 50
SEQ ID
GVKAENIIIETVPGSFELPYGSKLFVEKQKRLGKPLDAIIPIGVLIKGS
NO: 15
TMHFEYICDSTTHQLMKLNFELGIPVIFGVLTCLTDEQAEARAGLIE
GKMHNHGEDWGAAAVEMATKFN
I52-33B
MGANWYLDNESSRLSFTSTKNADIAEVHRFLVLHGKVDPKGLAEV
I52-33B:
SEQ ID
EVETESISTGIPLRDMLLRVLVFQVSKFPVAQINAQLDMRPINNLAP
61, 63, 66, 67, 72, 147, 148, 154, 155
NO: 16
GAQLELRLPLTVSLRGKSHSYNAELLATRLDERRFQVVTLEPLVIHA
QDFDMVRAFNALRLVAGLSAVSLSVPVGAVLIFTAR
I32-06A
MTDYIRDGSAIKALSFAIILAEADLRHIPQDLQRLAVRVIHACGMVD
I32-06A: 9, 12, 13, 14, 20, 30, 33, 34
SEQ ID
VANDLAFSEGAGKAGRNALLAGAPILCDARMVAEGITRSRLPADN
NO: 17
RVIYTLSDPSVPELAKKIGNTRSAAALDLWLPHIEGSIVAIGNAPTAL
FRLFELLDAGAPKPALIIGMPVGFVGAAESKDELAANSRGVPYVIVR
GRRGGSAMTAAAVNALASERE
I32-06B
MITVFGLKSKLAPRREKLAEVIYSSLHLGLDIPKGKHAIRFLCLEKED
I32-06B:
SEQ ID
FYYPFDRSDDYTVIEINLMAGRSEETKMLLIFLLFIALERKLGIRAHD
24, 71, 73, 76, 77, 80, 81, 84, 85, 88,
NO: 18
VEITIKEQPAHCWGFRGRTGDSARDLDYDIYV
114, 118
I32-19A
MGSDLQKLQRFSTCDISDGLLNVYNIPTGGYFPNLTAISPPQNSSIVG
I32-19A:
SEQ ID
TAYTVLFAPIDDPRPAVNYIDSVPPNSILVLALEPHLQSQFHPFIKITQ
208, 213, 218, 222, 225, 226, 229,
NO: 19
AMYGGLMSTRAQYLKSNGTVVFGRIRDVDEHRTLNHPVFAYGVGS
233
CAPKAVVKAVGTNVQLKILTSDGVTQTICPGDYIAGDNNGIVRIPVQ
ETDISKLVTYIEKSIEVDRLVSEAIKNGLPAKAAQTARRMVLKDYI
I32-19B
MSGMRVYLGADHAGYELKQAIIAFLKMTGHEPIDCGALRYDADDD
I32-19B:
SEQ ID
YPAFCIAAATRTVADPGSLGIVLGGSGNGEQIAANKVPGARCALAW
20, 23, 24, 27, 117, 118, 122, 125
NO: 20
SVQTAALAREHNNAQLIGIGGRMHTLEEALRIVKAFVTTPWSKAQR
HQRRIDILAEYERTHEAPPVPGAPA
I32-28A
MGDDARIAAIGDVDELNSQIGVLLAEPLPDDVRAALSAIQHDLFDL
I32-28A:
SEQ ID
GGELCIPGHAAITEDHLLRLALWLVHYNGQLPPLEEFILPGGARGAA
60, 61, 64, 67, 68, 71, 110, 120, 123,
NO: 21
LAHVCRTVCRRAERSIKALGASEPLNIAPAAYVNLLSDLLFVLARVL
124, 128
NRAAGGADVLWDRTRAH
I32-28B
MILSAEQSFTLRHPHGQAAALAFVREPAAALAGVQRLRGLDSDGE
I32-28B:
SEQ ID
QVWGELLVRVPLLGEVDLPFRSEIVRTPQGAELRPLTLTGERAWVA
35, 36, 54, 122, 129, 137, 140, 141,
NO: 22
VSGQATAAEGGEMAFAFQFQAHLATPEAEGEGGAAFEVMVQAAA
144, 148
GVTLLLVAMALPQGLAAGLPPA
I53-40A.1
MTKKVGIVDTTFARVDMASAAILTLKMESPNIKIIRKTVPGIKDLPV
I53-40A:
SEQ ID
ACKKLLEEEGCDIVMALGMPGKKEKDKVCAHEASLGLMLAQLMT
20, 23, 24, 27, 28, 109, 112, 113, 116,
NO: 23
NKHIIEVFVHEDEAKDDAELKILAARRAIEHALNVYYLLFKPEYLTR
120, 124
MAGKGLRQGFEDAGPARE
I53-40B.1
MDDINNQLKRLKVIPVIAIDNAEDIIPLGKVLAENGLPAAEITFRSSA
I53-40B: 47, 51, 54, 58, 74, 102
SEQ ID
AVKAIMLLRSAQPEMLIGAGTILNGVQALAAKEAGADEVVSPGFNP
NO: 24
NTVRACQIIGIDIVPGVNNPSTVEQALEMGLTTLKFFPAEASGGISM
VKSLVGPYGDIRLMPTGGITPDNIDNYLAIPQVLACGGTWMVDKKL
VRNGEWDEIARLTREIVEQVNP
I53-47A.1
MPIFTLNTNIKADDVPSDFLSLTSRLVGLILSKPGSYVAVHINTDQQL
I53-47A: 22, 25, 29, 72, 79, 86, 87
SEQ ID
SFGGSTNPAAFGTLMSIGGIEPDKNRDHSAVLFDHLNAMLGIPKNR
NO: 25
MYIHFVNLNGDDVGWNGTTF
I53-
MPIFTLNTNEKADDVPSDFLSLTSRLVGLILSEPGSYVAVHINTDQQL
I53-47A: 22, 25, 29, 72, 79, 86, 87
47A.1NegT2
SFGGSTNPAAFGTLMSIGGIEPDKNEDHSAVLFDHLNAMLGIPKNR
SEQ ID
MYIHFVDLDGDDVGWNGTTF
NO: 26
I53-47B.1
MNQHSHKDHETVRIAVVRARWHADIVDACVEAFEIAMAAIGGDRF
I53-47B:
SEQ ID
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEFV
28, 31, 35, 36, 39, 131, 132, 135, 139,
NO: 27
ASAVIDGMMNVQLDTGVPVLSAVLTPHRYRDSDEHHRFFAAHFAV
146
KGVEAARACIEILNAREKIAA
I53-
MNQHSHKDHETVRIAVVRARWHADIVDACVEAFEIAMAAIGGDRF
I53-47B:
47B.1NegT2
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVDGGIYDHEFV
28, 31, 35, 36, 39, 131, 132, 135, 139,
SEQ ID
ASAVIDGMMNVQLDTGVPVLSAVLTPHEYEDSDEDHEFFAAHFAV
146
NO: 28
KGVEAARACIEILNAREKIAA
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVP
I53-50A: 25, 29, 33, 54, 57
SEQ ID
DADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLD
NO: 29
EEISQFCKEKGVFYMPGVMTPTELVKAMKLGHDILKLFPGEVVGPQ
FVKAMKGPFPNVKFVPTGGVNLDNVCEWFKAGVLAVGVGDALVK
GDPDEVREKAKKFVEKIRGCTE
I53-
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVP
I53-50A: 25, 29, 33, 54, 57
50A.1NegT2
DADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLD
SEQ ID
EEISQFCKEKGVFYMPGVMTPTELVKAMKLGHDILKLFPGEVVGPE
NO: 30
FVEAMKGPFPNVKFVPTGGVDLDDVCEWFDAGVLAVGVGDALVE
GDPDEVREDAKEFVEEIRGCTE
I53-
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVP
I53-50A: 25, 29, 33, 54, 57
50A.1PosT1
DADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLD
SEQ ID
EEISQFCKEKGVFYMPGVMTPTELVKAMKLGHDILKLFPGEVVGPQ
NO: 31
FVKAMKGPFPNVKFVPTGGVNLDNVCKWFKAGVLAVGVGKALV
KGKPDEVREKAKKFVKKIRGCTE
I53-50B.1
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDIGGDRF
I53-50B:
SEQ ID
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEFV
24, 28, 36, 124, 125, 127, 128, 129,
NO: 32
ASAVIDGMMNVQLDTGVPVLSAVLTPHRYRDSDAHTLLFLALFAV
131, 132, 133, 135, 139
KGMEAARACVEILAAREKIAA
I53-
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDIGGDRF
I53-50B:
50B.1NegT2
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVDGGIYDHEFV
24, 28, 36, 124, 125, 127, 128, 129,
SEQ ID
ASAVIDGMMNVQLDTGVPVLSAVLTPHEYEDSDADTLLFLALFAV
131, 132, 133, 135, 139
NO: 33
KGMEAARACVEILAAREKIAA
I53-
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDIGGDRF
I53-50B:
50B.4PosT1
AVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFVVNGGIYRHEFV
24, 28, 36, 124, 125, 127, 128, 129,
SEQ ID
ASAVINGMMNVQLNTGVPVLSAVLTPHNYDKSKAHTLLFLALFAV
131, 132, 133, 135, 139
NO: 34
KGMEAARACVEILAAREKIAA
2. The recombinant nucleic acid of claim 1 , wherein the polypeptide is identical at least at half of the identified interface positions to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
3. The recombinant nucleic acid of claim 1 , wherein the polypeptide is identical at all of the identified interface positions to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
4. The recombinant nucleic acid of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical over its length to the sequence of a polypeptide selected from the group consisting of SEQ ID NO:1-34.
5. The recombinant nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS: 1-40.
6. A recombinant expression vector comprising the recombinant nucleic acid of claim 1 operatively linked to a promoter.
7. A recombinant host cell, comprising the recombinant expression vectors of claim 6 .
8. A recombinant expression vector comprising the recombinant nucleic acid of claim 2 operatively linked to a promoter.
9. A recombinant host cell, comprising the recombinant expression vectors of claim 8 .
10. A recombinant expression vector comprising the recombinant nucleic acid of claim 3 operatively linked to a promoter.
11. A recombinant host cell, comprising the recombinant expression vectors of claim 10 .
12. A recombinant expression vector comprising the recombinant nucleic acid of claim 4 operatively linked to a promoter.
13. A recombinant host cell, comprising the recombinant expression vectors of claim 12 .
14. A recombinant expression vector comprising the recombinant nucleic acid of claim 5 operatively linked to a promoter.
15. A recombinant host cell, comprising the recombinant expression vectors of claim 14 .