Near-infrared ray exposure system for biological studies
View Patent ↗An apparatus and methods of using the same for conducting photonic and optical treatments on biological samples with additional functions including temperature monitoring and real-time microscopic imaging are provided. The photonic and optical treatments can be conducted using light with wavelengths in the near-infrared region (NIR) on biological samples, including in-vitro brain cell cultures, in-vivo central nervous system (CNS) and peripheral nervous system (PNS) tissue samples, and other body tissues. The apparatus and methods can be combined with magnetic nanoparticles treatment to accomplish non-invasive, on-demand drug targeting, brain cell specific gene delivery, and magnetized photo-biomodulation for treating various CNS disorders.
1. An apparatus comprising:
a light source;
a cell culture plate configured to fix thereon a biological sample;
a sample stage having fixed thereon the cell culture plate and configured to provide heat to adjust a temperature of the sample;
a microscope apparatus capable of capturing and processing real-time images of the sample as it is subjected to the light emitted from the light source, the microscope apparatus comprising a microscope selected from an electron microscope and a scanning probe microscope;
a temperature-sensing device connected to the sample stage and measuring a temperature of the sample stage;
a magnetic coil configured to provide the sample stage with a magnetic force; and
an optical fiber connecting the light source to the microscope apparatus,
the light source and the microscope being disposed above the same top surface of the sample stage, and the light source being configured such that it serves as an incident light of the microscope,
the magnetic coil being disposed below the sample stage,
the temperature-sensing device being configured to monitor the real-time temperature of the sample as it is fixed on or with the sample stage,
the temperature-sensing device being selected from a thermometer, a thermistor, and a thermocouple,
the light source being positioned to directly face an upper surface of the cell culture plate,
the microscope apparatus further comprising computer hardware and software capable of capturing and processing real-time images of the biological sample, and
the light source being a near-infrared (NIR) light source, and the NIR light of the NIR light source having a wavelength in a range of from 700 nm to 1000 nm, and the NIR light having a power density in a range of from 1 W/cm 2 to 10 W/cm 2 .
2. The apparatus according to claim 1 , the biological sample comprising brain cells selected from human primary astrocytes, SK-N-MC human neuroepithelioma cells, CHME-5 human microglia cells, and a combination thereof.
3. The apparatus according to claim 1 , the light source emitting intermittent light waves.
4. The apparatus according to claim 1 , the light source emitting continuous light waves.
5. The apparatus according to claim 1 , the temperature-sensing device being a thermocouple.
6. The apparatus according to claim 1 , the biological sample being a sample treated with magnetic nanoparticles encapsulated by a light sensitive material.