Microfluidic device for storage and well-defined arrangement of droplets
The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time-resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
1. A device for storing cells comprising:
a plurality of storage channels arranged in series wherein each storage channel comprises one or more pots each comprising a constriction having a smallest cross-sectional dimension perpendicular to fluid flow through the plurality of storage channels that is smaller than an average cross-sectional dimension of a single cell; and
one or more bypass channels bypassing one or more of the plurality of storage channels,
wherein each pot has no more than two constrictions and is in direct fluid communication with no more than two other pots.
2. The device of claim 1 , wherein the volumes of the one or more pots are about 10 nanoliters.
3. The device of claim 1 , further comprising:
a gas-permeable membrane that separates the contents of the one or more pots from a controlled environment.
4. The device of claim 1 , further comprising:
a gas-permeable membrane that separates the contents of the one or more pots from ambient atmosphere.
5. The device of claim 1 , further comprising a thermal cycler operably associated with the one or more pots.
6. The device of claim 1 , wherein each pot is connected to exactly two constrictions.