Compositions for controlling
An isolated nucleic acid agent is disclosed comprising a nucleic acid sequence which downregulates expression of a gene product of a Varroa destructor mite. Compositions comprising same and uses thereof are also disclosed.
1. A composition comprising at least one nucleic acid agent which comprises a nucleic acid sequence which downregulates expression of a gene product of a Varroa destructor mite, and wherein said nucleic acid agent does not comprise a permeation enhancing agent selected from the group consisting of a virus, a polyamine, a polycation, a lipopolyamine, an anionic lipid, a neutral lipid, a pH sensitive lipid, transferrin, biotin, a serum protein, a glycoprotein, polyethylene glycol, and a cationic lipid.
2. The composition of claim 1 , wherein said at least one nucleic acid agent comprises at least five nucleic acid agents, for down-regulating ATPase subunit A, RNA polymerase III, Inhibitor of apoptosis (IAP), FAS apoptotic inhibitor and α-Tubulin, each of said at least five nucleic acid agent targeting a different gene.
3. The composition of claim 1 , wherein said at least one nucleic acid agent comprises at least six nucleic acid agents, for down-regulating ATPase subunit A, RNA polymerase I, RNA polymerase III, Inhibitor of apoptosis (IAP), FAS apoptotic inhibitor and α-Tubulin, each of said at least six nucleic acid agents for targeting a different gene.
4. The composition of claim 2 , wherein said nucleic acid agents are as set forth in SEQ ID Nos: 1, 13, 27, 30 and 39.
5. The composition of claim 3 , wherein said nucleic acid agents are as set forth in SEQ ID Nos: 1, 4, 7, 10, 13, 16, 19, 22, 25, 27, 30, 33, 36 and 39.
6. The composition of claim 1 , wherein said composition is in solid form.
7. The composition of claim 1 , wherein said composition is in liquid form.
8. The composition of claim 1 , wherein said composition comprises protein.
9. The composition of claim 7 , wherein said liquid is a sucrose solution.
10. The composition of claim 7 , wherein said liquid is a corn syrup solution.
11. The composition of claim 7 , wherein said liquid further comprises a carbohydrate or sugar supplement.
12. The composition of claim 1 , wherein said nucleic acid sequence comprises a sequence complementary to at least 21 nucleotides of Varroa destructor mite messenger RNA (mRNA).
13. The composition of claim 12 , wherein said Varroa destructor mite mRNA encodes a polypeptide selected from the group consisting of ATPase subunit A, RNA polymerase III, IAP, FAS apoptotic inhibitor, and α-Tubulin.
14. The composition of claim 1 , wherein said nucleic acid sequence is greater than 15 base pairs in length.
15. The composition of claim 1 , wherein said nucleic acid sequence is greater than 19 to 25 base pairs in length.
16. The composition of claim 1 , wherein said nucleic acid sequence is greater than 30 base pairs in length.
17. The composition of claim 1 , wherein said at least one nucleic acid agent is selected from the group consisting of a double-stranded RNA (dsRNA), an antisense RNA, and a ribozyme.
18. The composition of claim 17 , wherein said dsRNA is selected from the group consisting of a small inhibitory RNA (siRNA), a short-hairpin RNA (shRNA), and a microRNA (miRNA).