Beta lactamase as biomarker for the specific detection of tuberculosis-complex bacteria
The present disclosure provides methods, reagents, systems, and devices that target β lactamase as a biomarker for the sensitive and specific detection of tuberculosis-complex bacteria. Specifically, the present disclosure relates to methods and compositions for the detection of specific β-lactamase protein and nucleic acid sequences to indicate the presence of tuberculosis-complex bacteria.
1. A method of detecting the presence of tuberculosis-complex bacteria in a biological sample, the method comprising contacting the sample with an antibody or antibody fragment that specifically binds to a β-lactamase (BlaC) of a tuberculosis-complex bacteria, and detecting the formation of a complex between the BlaC and the antibody or antibody fragment, wherein the formation of a complex is indicative of the presence of tuberculosis-complex bacteria in the sample.
2. The method of claim 1 , wherein the antibody or antibody fragment specifically binds to BlaC with an amino acid sequence set forth in SEQ ID NO:2.
3. The method of claim 1 , wherein the antibody or antibody fragment comprises a detectable label.
4. The method of claim 1 , wherein the formation of a complex between the BlaC and the antibody or antibody fragment is detected by further contacting the complex with an affinity reagent that contains a detectable label and that specifically binds to the complex.
5. The method of claim 1 , further comprising contacting the biological sample with an immobilized BlaC protein or immobilized antibody or antibody fragment.
6. The method of claim 1 , wherein the biological sample is obtained from a subject suspected of having tuberculosis-complex bacteria, and wherein the presence of tuberculosis-complex bacteria in the biological sample is indicative of a tuberculosis infection in the subject.
7. The method of claim 6 , wherein the subject is human.
8. The method of claim 1 , wherein the antibody is a polyclonal antibody, a monoclonal antibody, a single chain antibody, an antigen binding enzymatic digestion product of the antibody, a chimeric antibody, or a humanized antibody.
9. The method of claim 1 , wherein the tuberculosis-complex bacteria are from one or more of the species selected from the group consisting of: Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis - Bacillus Calmette-Guérin (BCG), Mycobacterium africanum, Mycobacterium microti, Mycobacterium canettii, Mycobacterium pinnipedii , and Mycobacterium mungi.