Methods, devices, and systems for microfluidic stress emulation

1. A method for monitoring platelet activation state in a subject, the method comprising:

a) modeling the shear stress profile of a pathology in a medical device,

wherein the pathology is a cardiovascular pathology,

wherein the medical device is a mechanical circulatory support device,

wherein the modeling comprises one or more of device thrombogenicity emulation, producing a probability density function of the medical device, and determining individual particle trajectories for particles flowing through the medical device;

b) reproducing the shear stress profile of the pathology in the device in a microfluidic device;

c) flowing a sample from the subject through the microfluidic device, wherein the sample comprises platelets separated from a blood sampled obtained from the subject,

wherein the sample has a volume of 1 to 100 nanoliters, 1 to 1000 nanoliters, 1 to 100 microliters, or 1 to 1000 microliters; and

d) testing a platelet activation state of the sample.

2. The method of claim 1 wherein the pathology is a deviation from a normal geometry or flow characteristic.

3. The method of claim 1 wherein the pathology is an arterial narrowing, stenosis, outpouching, anurysm or pseudoaneurysm, dissection, flap, fistula, constriction, thrombus, intinal hyperplasia, or tumor.

4. The method of claim 1 wherein the medical device is a ventricular assist device, stent, total artificial heart, or valve.

5. The method of claim 1 further comprising adjusting the dose of a drug or other therapeutic agent administered to the subject and/or adjusting the dose of an anti-thrombosis drug administered to the subject.

6. The method of claim 1 wherein the sample is acquired from the patient 1 to 10 times a day.