Matrix for restoring soft tissue and producing method therefor
Disclosed are a matrix for restoring soft tissue and a method of producing the same, the method including: forming a sheet-type absorption layer ( 11 ) on one side of a double layer using biocompatible neutral collagen; forming a film-type support layer ( 12 ) on the remaining surface of the double layer by mixing collagen with a water-soluble polymer and a natural material; and forming a double layer-structured matrix ( 10 ) for restoring soft tissue by attaching the absorption layer ( 11 ) and the support layer ( 12 ) so as to treat damage to soft tissue of a mammal other than a human or restore damaged soft tissue. This invention pertains to the treatment of damage to soft tissue such as tendons, ligaments, and rotator cuffs or the restoration of damaged soft tissue, whereby the quality and reliability of products can be remarkably improved, thus satisfying various needs of consumers, who are the users thereof, and exhibiting a good effect.
1. A method of producing a double layer-structured matrix for treating or restoring damaged soft tissue, the method comprising:
forming a sheet-type absorption layer using biocompatible neutral collagen, wherein the neutral collagen has pH of 7.0, and the sheet-type absorption layer is porous;
forming a film-type support layer by mixing collagen with a water-soluble polymer and a natural material comprising alginate; and
forming the double layer-structured matrix by attaching the absorption layer and the support layer,
wherein the forming of the sheet-type absorption layer comprises:
preparing a neutral collagen solution using collagen having a concentration of 0.5 to 8.0% (w/w);
gelling the neutral collagen solution using glutaraldehyde as a crosslinking agent;
forming a sheet by lyophilizing the gelled neutral collagen;
subjecting the formed sheet to dehydrothermal treatment; and
pressing the formed sheet subjected to the dehydrothermal treatment to a predetermined thickness,
wherein the forming of the film-type support layer comprises:
dissolving and mixing a collagen solution and the water-soluble polymer at a ratio of 1:7 to 7:9, together with the natural material comprising alginate to prepare a mixed solution; and
crosslinking the mixed solution by placing the mixed solution in a mold, defoaming the mixed solution using a decompressor, and sealing and gamma-crosslinking the defoamed mixed solution at a gamma irradiation dose of 5 to 40 kGy, and
wherein the sheet-type absorption layer has a thickness of 0.4 to 0.5 mm and the film-type support layer has a thickness of 0.2 mm or less.
2. The method of claim 1 , wherein the collagen having the concentration of 0.5 to 8.0% (w/w) is prepared using purified water, and is then made into the neutral collagen solution having a pH of 7.0 using NaOH.
3. The method of claim 1 , wherein the neutral collagen solution is stirred using a stirrer for 80 minutes or more in a reaction tank at 4° C. or less.
4. The method of claim 1 , wherein the glutaraldehyde is used in an amount of 0.4 ml or less of 50% w/w glutaraldehyde for 1 g of collagen.
5. The method of claim 1 , wherein the neutral collagen solution containing the crosslinking agent is crosslinked at a refrigeration temperature for 2 hours or more.
6. The method of claim 1 , wherein 162 to 198 g of the crosslinked neutral collagen solution is aliquoted in a square dish having a size of 230×230 mm, reacted at a refrigeration temperature for 24 hours or more and then gelled at room temperature for 4 hours or more and thus crosslinked.
7. The method of claim 1 , wherein the subjecting the formed sheet to dehydrothermal treatment is performed in a manner in which the gelled neutral collagen is lyophilized in a vacuum using a dry oven at a temperature of −20° C. or less for 4 hours or more to make a sponge-type sheet, which is then pressed, thus obtaining a lyophilized sheet.
8. The method of claim 1 , wherein the collagen is aseptically prepared in a manner in which 5 mg/mL or less of collagen is sterilized using a 0.22 μm filter and then concentrated through aseptic manipulation, and a concentration of the collagen for use in the filter ranges from 5 to 100 mg/mL.
9. The method of claim 1 , wherein the dissolving and mixing is performed in a manner in which, for the collagen solution, 0.5 to 2% (w/v) of a sterilized biocompatible collagen powder is mixed with a 0.1 M HCl solution having a pH of 3.0 to 4.0 and is then stirred for 24 hours, and for the water-soluble polymer, 3 to 10% (w/v) of the water-soluble polymer is dissolved in water at 30° C. or more.
10. The method of claim 1 , wherein the collagen is used in an amount of 0.3 to 1.0% (w/v) and the water-soluble polymer is used in an amount of 0.9 to 2.5% (w/v).
11. The method of claim 1 , wherein the water-soluble polymer comprises one or more selected from the group consisting of polyvinyl alcohol, polyvinyl pyrrolidone, and polyethylene glycol.
12. The method of claim 1 , wherein the crosslinking is performed through physical crosslinking or chemical crosslinking.
13. The method of claim 9 , wherein the biocompatible collagen powder is prepared into a 1.0% (w/v) acidic collagen aqueous solution, a 3.0% (w/v) water-soluble polymer aqueous solution is prepared, and the two aqueous solutions are mixed so that an amount of the collagen is 0.3 to 0.7% (w/v) and an amount of the water-soluble polymer is 0.9 to 2.5% (w/v) based on a total weight thereof to form a mixed solution, which is then gamma-crosslinked at a gamma irradiation dose of 5 to 40 kGy, thus forming the film-type support layer.