Compositions and methods for the diagnosis of lyme disease
This disclosure provides antigen compositions useful for diagnosing Lyme disease and for detecting antibodies that bind to Borrelia antigens. The antigenic compositions comprise a mixture of hybrid peptides and Borrelia proteins. This disclosure also provides devices, methods, and kits that are useful for diagnosing Lyme disease and for detecting anti- Borrelia antibodies in a sample to aid in the diagnosis of Lyme disease.
1. A method of detecting antibodies to Lyme disease in a sample from a subject suspected of having Lyme disease, the method comprising:
contacting the sample with three capture moieties and a detection moiety; and detecting a signal from the detection moiety,
wherein one of the three capture moieties is a BBA25 (Decorin binding protein B) protein attached to a solid support, one is a BB0147 (Flagellar filament (FlaB)) protein attached to a solid support, and one is a hybrid peptide consisting of amino acid sequence SEQ ID NO:1 attached to a solid support, and each is capable of forming at least one complex with at least one antibody to a Borrelia antigen in the sample,
wherein the detection moiety binds to the antibody of the antibody-capture moiety complex,
wherein the formation of the at least one complex is indicative of the presence of an anti-Lyme antibody to the Borrelia antigen in the sample, and
wherein the amount of signal detected is proportional to the anti-Lyme antibody in the sample.
2. The method of claim 1 , wherein the detection moiety is capable of detecting immunoglobulin G (IgG).
3. The method of claim 1 , wherein the detection moiety is capable of detecting immunoglobulin M (IgM).
4. The method of claim 1 , wherein the detection moiety comprises a binding partner and a label, and wherein the label is selected from the group consisting of electrochemiluminescence labels or compounds, chemiluminescent compounds, enzyme labels, fluorophores, chromogenic compounds, radiolabels, catalysts, colorimetric, labeled antibodies a latex particle, a magnetic particle, a radioactive element, fluorescent dyes, phosphorescent dyes, dye crystalites, gold particles, silver colloidal particles, selenium colloidal particles, metal chelates, coenzymes, electro active groups, oligonucleotides, and stable radicals.
5. The method of claim 4 , wherein the metal chelate comprises a ruthenium or an osmium metal chelate.
6. The method of claim 1 , wherein the Borrelia antigen comprises an antigen from a Borrelia afzelii, Borrelia burgdotieri, Borrelia garinii, Borrelia miyamotoi , or Borrelia valaisiana species.
7. The method of claim 1 , wherein the solid support comprises a bead, a superparamagnetic bead, a paramagnetic bead, a plate, a glass surface, a plastic surface, a metal surface, a polystyrene surface, a nitrocellulose surface, a microparticle, a nano-particle surface, a flow path in a lateral flow assay device, or a well in a microtiter plate.
8. The method of claim 1 , wherein the detecting step comprises performing an ELISA assay, a lateral flow assay, a fluorescence assay, or an electrochemiluminescence assay.
9. The method of claim 1 , wherein the detecting step comprises performing an electrochemiluminescence assay.
10. The method of claim 1 , wherein the sample comprises a human sample, a canine sample, or an equine sample.
11. The method of claim 1 , wherein the sample comprises a blood, a serum, a plasma, a cerebrospinal fluid, a urine, or a saliva sample.