Granted Patent B2

US 12,638,446 · App. 18/814,445 · Granted May 26, 2026

Inventors: Monica Embers (New Orleans, LA); Tammy Crawford (Paradise Valley, AZ); Holly Ahern (Charlton, NY)

Assignees: Focus on Lyme Foundation; The Administrators of The Tulane Educational Fund

G01N33/56911

Abstract

Claims (24)

1 . A set of reagents to measure the levels of biomarkers in a specimen, wherein the biomarkers are a binding molecules each binding molecule bound to an imaging agent, each binding molecule capable of binding one antigen in a panel of antigens, the panel selected from the group consisting of the following panels and each panel consisting of the specified antigens or measurable fragments thereof:

(a) outer surface protein C2 (OspC2), and an 26-amino acid peptide within the VIsE protein (C6);

(b) C6, glycerophosphodiester phosphodiesterase protein (GlpQ), protein p35 (p35), porin protein p66 or the protein p66 (p66), outer surface protein C (OspC) decorin-binding protein A (DbpA), periplasmic oligopeptide binding protein (OppA2), protein exported repeated protein Q (ErpQ), outer surface protein E (OspE), OspC2;

(c) C6, p35, p66, OspC, DbpA, OppA2, ErpQ, OspE, OspC2;

(d) OspC2, OspC, C6, and p35;

(e) OspC2, OspC, C6, and p66;

(f) OspC2, OspC, C6, and DbpA;

(g) OspC2, OspC, C6, and OppA2;

(h) OspC2, OspC, Co, and ErpQ; and

(i) OspC2, OspC, C6, and OspE.

2 . The set of reagents of claim 1 , wherein the reagents are binding molecules.

3 . The set of reagents of claim 2 , wherein the binding molecules are antibodies.

4 . A test kit comprising the set of reagents of claim 1 .

5 . A multianalyte panel assay comprising the set of reagents of claim 1 .

6 . A set of antigens fixed to a plurality of beads to measure the levels of biomarkers in a specimen, wherein the biomarkers are antibodies each antibody bound to an imaging agent, each antibody capable of binding one antigen in a panel of antigens selected from the group consisting of the following panels and each panel consisting of the specified antigens or measurable fragments thereof:

(a) OspC2, and C6;

(b) C6, GlpQ, p35, p66, OspC, DbpA, OppA2, ErpQ, OspE, OspC2;

(c) C6, p35, p66, OspC, DbpA, OppA2, ErpQ, OspE, OspC2;

(d) OspC2, OspC, Co, and p35;

(e) OspC2, OspC, C6, and p66;

(f) OspC2, OspC, C6, and DbpA;

(g) OspC2, OspC, C6, and OppA2;

(h) OspC2, OspC, C6, and ErpQ; and

(i) OspC2, OspC, C6, and OspE.

Assignments (1)

Continuity (2)

References Cited (285)