Granted Patent B2

US 12,631,635 · App. 17/248,236 · Granted May 19, 2026

Inventors: Jyotsna S. Shah (Santa Clara, CA); Song Liu (San Jose, CA)

Assignee: ID-Fish Technology, Inc.

G01N33/56911C07K14/20C07K2319/40G01N2333/20G01N2469/20

Abstract

Claims (38)

1 . A composition comprising one or more labeled and/or bound amino acid sequences selected from the group consisting of:

SEQ ID NO: 1;

SEQ ID NO: 2;

SEQ ID NO: 3;

SEQ ID NO: 4;

SEQ ID NO: 5 and variants thereof having at least 90% homology;

SEQ ID NO: 6 and variants thereof having at least 90% homology;

SEQ ID NO: 7;

SEQ ID NO: 8;

SEQ ID NO: 9;

SEQ ID NO: 10; and

SEQ ID NO: 11 and variants thereof having at least 96% homology;

wherein, when labeled, the one or more amino acid sequences are labeled with biotin/streptavidin, enzyme conjugates, fluorescent moieties, or biological fluorophores,

wherein, when bound, the one or more amino acid sequences are bound to a substance selected from the group consisting of nitrocellulose, nylon, polyvinylidene difluoride (PVDF), magnetic beads, and agarose.

2 . A method for detecting antibodies resulting from infection by one or more members of Tick-Borne Relapsing Fever (TBRF) Borrelia genus if present in a biological sample from a subject suspected of having TBRF, the method comprising:

providing the biological sample obtained from the subject suspected of having TBRF;

contacting the biological sample with a composition comprising one or more labeled and/or bound amino acid sequences selected from the group consisting of:

SEQ ID NO: 1;

SEQ ID NO: 2;

SEQ ID NO: 3:

SEQ ID NO: 4;

SEQ ID NO: 5 and variants thereof having at least 90% homology;

SEQ ID NO: 6 and variants thereof having at least 90% homology;

SEQ ID NO: 7;

SEQ ID NO: 8;

SEQ ID NO: 9;

SEQ ID NO: 10; and

SEQ ID NO: 11 and variants thereof having at least 96% homology;

wherein, when labeled, the one or more amino acid sequences are labeled with biotin/streptavidin, enzyme conjugates, fluorescent moieties, or biological fluorophores,

wherein, when bound, the one or more amino acid sequences are bound to a substance selected from the group consisting of nitrocellulose, nylon, polyvinylidene difluoride (PVDF), magnetic beads, and agarose; and

detecting a positive immunobinding reaction which indicates the presence of one or more TBRF specific antibodies in the biological sample.

3 . The method of claim 2 , wherein the biological sample is considered positive for TBRF if at least two positive immunobinding reactions are detected.

4 . The method of claim 2 , wherein the positive immunobinding reaction is detected with anti-human IgG or anti-human IgM antibody linked to a detectable moiety.

5 . The method of claim 4 , wherein the detectable moiety is selected from the group consisting of chromophores, radioactive moieties and enzymes.

6 . The method of claim 4 , wherein the detectable moiety comprises alkaline phosphatase.

7 . The method of claim 4 , wherein the detectable moiety comprises biotin.

8 . The composition of claim 1 , wherein the variants of SEQ ID NOs: 5 and 6 have at least 95% homology.

9 . The composition of claim 8 , wherein the one or more labeled and/or bound amino acid sequences are selected from the group consisting of SEQ ID NOs: 1-11.

Assignments (1)

Continuity (1)

References Cited (26)