Rapid test for Lyme bacteria
View Patent ↗The subject matter disclosed herein provides a method and a device for detection of one or more bacteria in a sample.
1. A device for detecting Borrelia sp. in a sample comprising:
a holder with a top part comprising at least one sample opening and at least one observation opening and a continuous bottom part;
a top glass slide and a bottom glass slide disposed between the top part and the bottom part of said holder such that the holder encases the top glass slide and the bottom glass slide;
at least one sample pad disposed on top of the bottom glass slide such that at least a portion of the at least one sample pad is not covered by the top glass slide and at least a portion of the at least one sample pad is in contact with a first short edge of at least one elongated nitrocellulose strip and at least a portion of the sample pad aligns with at least one sample opening on the top part of the holder;
wherein the at least one sample opening is shaped to receive a pestle for homogenization of the sample on the sample pad and is configured to constrain movement of the sample on the sample pad during homogenization of the sample following application thereof to the sample pad;
wherein a first portion of said at least one nitrocellulose strip is pre-loaded with at least one variable major protein (VMP)-like sequence (Vls) E lipoprotein (VlsE) C6 peptide-specific antibody and a second portion of the at least one nitrocellulose strip is pre-loaded with biotinylated streptavidin;
further wherein the at least one nitrocellulose strip is disposed between the top glass slide and the bottom glass slide; and
at least one cellulose wick disposed in contact with a second short edge of the at least one nitrocellulose strip.
2. The device of claim 1 , wherein the device is configured to detect Borrelia burgdorferi in the sample following application of the sample to the sample pad.
3. The device of claim 1 , wherein the first portion of the nitrocellulose strip comprises a first read out-line comprising the at least one C6 peptide-specific antibody.
4. The device of claim 3 , wherein the device is configured so that the read-out line does not change color if Borrelia sp. is detected in the sample.
5. The device of claim 3 , wherein the device is configured so that the read-out line changes color if Borrelia sp. is detected in the sample.
6. The device of claim 1 , wherein the sample comprises a tick or a portion thereof.