IP Library › Granted Patent US 10,928,394
Granted Patent B2
US 10,928,394 · App. 16/422,802 · Granted Feb 23, 2021

Rapid test for lyme bacteria

Inventor: Julian R. K. Kage (Glastonbury, CT)
G01N33/56911G01N33/54386
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Quick Facts
Patent No.
US 10,928,394
App. No.
16/422,802
Granted
Feb 23, 2021
Kind
B2
Abstract

The subject matter disclosed herein provides a method and a device for detection of one or more bacteria in a sample.

Claims (9)

1. A method for detecting Borrelia burgdorferi bacteria in a sample, the method comprising:

homogenizing a sample on a sample pad using a pestle;

applying biotinylated C6 peptide, streptavidin-gold, and flow buffer to the homogenized sample to obtain a homogenized sample solution;

allowing for capillary flow of said homogenized sample solution to occur along an elongated nitrocellulose strip contacting said sample pad at a first short edge;

allowing the homogenized sample solution to contact a first read-out line on the nitrocellulose strip and a second read-out line on the nitrocellulose strip;

wherein the first read-out line comprises at least one VisE-specific antibody;

further wherein the second read-out line comprises biotinylated albumin; and

collecting excess sample in a cellulose wick contacting a second short edge of said nitrocellulose strip;

wherein: (1) the first read out line does not change color if bacterial is detected in the sample; or (2) the first read out line changes color if bacteria is not detected in the sample.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 14, 2025
From: KAGE, JULIAN
To: EXACTICS, INC.
Reel/Frame 071697/0319 →
Continuity (2)
Provisional Application 62802547 · Feb 7, 2019
Related Publication 20200256870A1 · Aug 13, 2020
Cited By (1)
US 12,638,446