Rapid test for lyme bacteria
View Patent ↗The subject matter disclosed herein provides a method and a device for detection of one or more bacteria in a sample.
1. A method for detecting Borrelia burgdorferi bacteria in a sample, the method comprising:
homogenizing a sample on a sample pad using a pestle;
applying biotinylated C6 peptide, streptavidin-gold, and flow buffer to the homogenized sample to obtain a homogenized sample solution;
allowing for capillary flow of said homogenized sample solution to occur along an elongated nitrocellulose strip contacting said sample pad at a first short edge;
allowing the homogenized sample solution to contact a first read-out line on the nitrocellulose strip and a second read-out line on the nitrocellulose strip;
wherein the first read-out line comprises at least one VisE-specific antibody;
further wherein the second read-out line comprises biotinylated albumin; and
collecting excess sample in a cellulose wick contacting a second short edge of said nitrocellulose strip;
wherein: (1) the first read out line does not change color if bacterial is detected in the sample; or (2) the first read out line changes color if bacteria is not detected in the sample.